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Lilac hospital > M2: Routine Histopathology Techniques > Flashcards

M2: Routine Histopathology Techniques Flashcards

1
Q

the tissue is then carefully dissected & examined under microscope whether stained or not

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

a. Teasing/Dissociation

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2
Q

a selected tissue specimen is immersed in a watch glass containing isotonic salt solution

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

a. Teasing/Dissociation

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3
Q

small pieces of tissues (not more than 1 mm) are placed in a slide & forcibly compressed with another slide or cover glass

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

b. Squash Preparation

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4
Q

cellular materials are spread lightly over a slide using a wire loop/applicator or by making an opposition smear with another slide

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

c. Smear Preparation

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5
Q

the material is spread rapidly and gently in a direct/zigzag line throughout the slide

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

d. Streaking

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6
Q

a selected portion is transferred to a clean glass slide and is gently spread moderately thick film by teasing the mucous strands apart using an app stick

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

e. Spreading

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7
Q

the 2 slides are then pulled apart with single, uninterrupted motion

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

f. Pull-apart

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8
Q

the surface of freshly cut piece of tissue is brought into contact and pressed on the surface of a clean glass slide

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

g. Touch Preparation

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9
Q

normally used when a rapid diagnosis of tissue in question is required especially recommended when lipids or nervous tissue elements are to be demonstrated

a. Teasing/Dissociation
b. Squash Preparation
c. Smear Preparation
d. Streaking
e. Spreading
f. Pull-apart
g. Touch Preparation
h. Frozen

A

h. Frozen

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10
Q

This is a hole on the microscope stage, through which the transmitted light from the source reaches the stage.

A

Aperture

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11
Q

These are lenses that are used to collect and focus light from the illuminator into the specimen. They are found under the stage next to the diaphragm of the microscope.

A

Condenser

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12
Q

It is also known as the iris. It is found under the stage of the microscope and itsprimary role is to control the amount of light that reaches the specimen.

A

Diaphragm

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13
Q

It controls how far the stages should go, preventing the objective lens from getting too close to the specimen slide that may damage the specimen. It is responsible for preventing the specimen slide from coming too far up and hit the objective lens.

A

Rack stop

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14
Q

The object appears dark against a bright background and the common microscope used in the laboratory is the compound microscope because it contains two types of lenses that function to magnify objects

A

Light or Brightfield Microscope

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15
Q

The object appears bright against a dark background

A

Darkfield Microscope

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16
Q

The energy source used in the electron microscope is a beam of electrons since the beam has an exceptionally short wavelength

A

Electron Microscope

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17
Q

The light microscope relies on differences either in color or in light absorption to provide the contrast that makes it possible for us to identify the objects under the microscope.

A

Phase Contrast Microscope

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18
Q

It is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image.

A

Phase Contrast Microscope

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19
Q

It is used in histology primarily for the identification of crystals, such as talc, silica, alum, hematein, and urates.

A

Polarizing Microscope

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20
Q

A tissue processor is a device that prepares tissue sample for sectioning by microscopic examination in the diagnostic laboratory.

A

Automatic Tissue Processor Machine

(Elliot Bench–Type Processor)

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21
Q

It is a machine specifically designed to cut very thin sections of tissue. It uses steel, glass or diamond plate depending upon the specimens to slice for the desire thickness of sections.

A

Microtome

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22
Q

prepare animals or plant tissues for light microscope in histology.

A

Steel Plates

23
Q

slice sections for light microscopy and to slice very thin sections for electron microscopy.

A

Glass Knives

24
Q

Plates that are used to slice hard material.

A

Diamond Knives

25
Q

it is used for cutting serial sections of large blocks of paraffin embedded tissues.

A

Rocking Microtome

26
Q

Invented Rocking Microtome in 1881

A

Paldwell Trefall

27
Q

It is the oldest type of microtome and it is cheap and simple. It is available in two sizes the small and large blocks of paraffin.

A

Rocking Microtome

28
Q

cut paraffin embedded tissues and is the most common type used for both routine and research laboratories at present.

A

Rotary (Minot) Microtome
(parrafin)

29
Q

for cutting celloidin embedded sections. This was developed by Adams in 1789. There are two types of this microtome, the base sledge microtome and standard sliding microtome.

A

Sliding Microtome

30
Q

cutting sections of very large blocks like whole brain commonly used in neuropathology and ophthalmic pathology and more stable that ordinary sliding microtome.

A

Base Sledge Microtome

31
Q

it is different from base sledge in that blocks remain stationary while knife is moved backward and forward during the process.

A

Standard Sliding Microtome

32
Q

for cutting unembedded frozen sections

A

Freezing Microtome
(queckett)

33
Q

for cutting sections for electron microscopy.
The ultrathin microtome is primarily used for cutting tissue sections at
0.5 micra, for electron microscopy. Knives used for cutting ultrathin sections are broken plate glass in
which preferred are the diamond knives.

A

Ultrathin Microtome

34
Q

A sharp knife in good condition is indispensable to the histologic technician and is the most important requirement for satisfactory cutting.

A

Microtome Knives

35
Q

is used to section paraffin, paraplast, carbowax embedded blocks.

A

Plane-Concave Knife

36
Q

used to cut celloidin-embedded block.

A

Planoconcave Knife

37
Q

used to cut frozen blocks.

A

Plane Wedge Knife

38
Q

a stone used to remove nicks from the knife

A

Hone

39
Q

leather strip used to remove the burrs created by honing

A

Strop

40
Q

for manual sharpening when cutting edge has been rendered blunt or nicked. This type usually gives the best result.

A

Belgium Yellow

41
Q

give more polishing effect than the Belgian yellow.

A

Arkansas

42
Q

is much coarser than the first two types and
is used only for badly nicked knives followed by either one of the first two sharpeners.

A

Fine Carborundum

43
Q

Honing for Biconcave knives

A

20-40 strokes

44
Q

Honing for Plane Wedge knives

A

10-20 Strokes

45
Q

process whereby the “burr” formed during honing is removed and the cutting edge of the knife polished.

A

Stropping

46
Q

Made from acetyl polymer. which is a special high-density polymer that keeps specimens safely submerged in solution.

A

Tissue Casette

47
Q

These are used to mold the tissue block for easier handling during cutting.

A

Embedding Molds

48
Q

It is used for infiltration of tissues in paraffin, paraplast, carbowax, plastic/resin. The temperature is set at the recommended amount depending on the medium used.

A

Oven

49
Q

If the oven is not available, it is used regulated at 37 degree Celsius, is a welcome piece of equipment in the pathology laboratory

A

Incubator
(an excellent drying oven.)

50
Q

to float, smoothen, flatten, and facilitate separation of embedded tissue ribbons.

A

Tissue Flotation Bath

51
Q

One consists of a during chamber within which a steady current of warm air blows over the slides and carries away the moisture.

A

Slide Dryers

52
Q

securely hold slides to stain cell components for better microscopic studies.

A

Staining Jars

53
Q

It used at a time to flatten and
contain the sample when
viewing section or smear, they
must be fixed to the slides.

A

Slides and Cover Slips

Lilac hospital (8 decks)

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