M2: ADDITIONAL VIDEO

Question 1

In the DNA replication fork which one from 3’ and 5’ is the leading and lagging strand ?

Answer 1

3’ leading strand

5’ lagging strand

Question 2

Process of DNA replication ?

Answer 2

DNA is first unwound from the helical duplex (helix)

The new strand is elongated by hydrogen bonding

A nucleophilic attack of the deoxyribose 3 ′ hydroxyl oxygen on a phosphorous atom of the phosphate group on the hydrogen-bonded nucleotide triphosphate.

Ortho phosphate is released

Consist of one template strand and one newly synthesized strand.

Question 3

catalyze the addition of methyl groups to nitrogen

bases, usually adenines and cytosines in DNA strands

Answer 3

methyltransferases

Question 4

Where is ρ factor (rho factor) used ?

Answer 4

transcription termination in other genes

Question 5

In eukaryotes, mRNA synthesis, catalyzed by pol II, proceeds along the DNA template until a ___ is encountered.

Answer 5

polyadenylation signal (polyA site)

Question 6

In transcription initiation the promoter recruits ___, a DNA binding protein, which in turn recruits other proteins.

Answer 6

TATA protein