M11 Gram (-ve part 2) Flashcards
list three gram negative anaerobic bacilli
tennerella
fusobacterium
leptotrichia
which saccharolytic, non-pigmented bacteria pits the surface of blood agar?
tannerella forsythia
which condition is a tanneralla forsythia an important part of?
periodontitis
which important virulence factors are associated with tannerella forsythia?
fibronectin/ fibrinogen binding BspA
Protease
what are the effects of BspA on tanerella forsythia?
adhesion
Induces release of bone reabsorbing pro-inflammatory cytokines
induces chemokines (recruits neutrophils, inflammation)
what are the interactions of BspA (Tannerella forsythia?
P.gingivalis enhances attachement to epithelial cells
- forms mixes films with fusobacterium
which GNAB is mainly found in human gingival sulcus?
fusobacterium nucleatum
which bacteria can adhere with Gm +ve and Gm -ve?
fusobacterium nucleatum
which virulence factor is gaining a lot of attention in extra-oral infections? (fusobacterium)
FadA
the spirochaete combination of Fusobacterium nucleatum and Treponema vincentii is?
fusospirochaetal infection
what is fusobacterium nucleatum associated with?
Acute ulcerative Gingivitis (AUG)
how does fusobacterium (mainly smokers) lead to AUG
- induces protein kinase (cell survival)
- stimulates collagenase 3 expression
- secretes serine protease
- immune suppression
which saccrolytic Gnab makes lactic acid as a major fermentation product?
leptotrichia -
which main species is preferentially isolated from plaque overlying caries?
leptotrichia buccalis (present in dental plaque_ - extra oral infections in seriously ill patients
What is the meaning of gram -ve facultative anaerobes?
Prefer oxygen and are often capnophilic
what are the 4 main oral species of facultative anaerobes?
Hemophilus
Aggregatibacter
Eikenella
capnocytophaga
which gram -ve small bacilli are found in the mouth in first few days of birth?
haemophilus
what is the most common haemophilus soecies found in supragingival plaque?
haemophilus segnis
describe aggregatibacter oral species
gram -ve
capnophilic
periodontipathogen
often found associated with actinomyces
what is juvenile periodontitis?
common in girls puberty
west african and asian fam
localised JP (first permanent molars and incisors)
which actinomyces is associated with Juvenile periodontitis?
A.actinomycetemcomitans
rapid destructive periodontitis
what anitbiotic is given for A.actinomycetemcomitans?
tertracycline and beta lactam
describe eikenella
gram -ve asaccharolytic small bacillus that pits or corrodes agar
what serious extra oral infection can eikenella cause?
brain and abdominal abscesses, endocarditis 25% of human bite/clench fist injuries
which curved gram -ve bacilli is found in plaque and periodontal pockets?
Campylobacter rectus
what is the red complex?
tennerella forsythia
porphyromonas gingivalis
treponema denticola
what is the orange complex made up of?
prevotella intermedia
prevotella nigrescens
fusobacterium nucleatum
