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M1 - LAB Flashcards

1
Q

ELECTROPHORESIS SEPERATION BASED ON

A

ELECTRICAL CHARGE DENSITY

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2
Q

MIGRATION OF CHARGED SOLUTES IN A MEDIUM UNDER ELECTRIC FIELD

A

ELECTROPHORESIS

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3
Q

ELECTROPHORESIS BUFFER

A

BARBITAL (PH 8.6)

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4
Q

MOLECULE WHOSE NET CHARGE CAN EITHER BE + OR -

A

AMPHOLYTE

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5
Q

IN ELECTROPHORESIS ___ ATTRACTS

A

OPPOSITE

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6
Q

MIGRATION OF SMALL IONS

A

IONTOPHORESIS

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7
Q

MIGGRATION OF CHARGED MACROMOLECULE IN POROUS MEDIUM

A

ZONE ELECTROPHPORESIS

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8
Q

ZE: SEPERATION BY MOLECULAR SIZE

A

CELLULOSE ACETATE

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9
Q

ZE: SEPERATION BY ELECTRICAL CHARGE

A

AGAROSE GEL

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10
Q

ZE: SEPERATION BY CHARGE AND MOLECULAR SIZE

A

POLYCRILAMIDE GEL

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11
Q

POLYCRILAMIDE GEL SEPERATES PROTEIN INTO __

A

20 FRACTIONS

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12
Q

DISPLAY OF PROTEIN ZONES

A

ELECTROPHORETOGRAM

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13
Q

FACTORS AFFECTING MIGRATION

A

TYPE OF SUPPORT MEDIUM
TEMP OF SUPPORT MEDIUM

MW OF PARTICLE
STRENGTH OF E. FIELF

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14
Q

THE DRIVING FORCE OF ELECTRO

A

POWER SUPPLY

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15
Q

FIVE COMPONENTS

A

POWER SUPPLY
SUPPORT MEDIUM
BUFFER
SAMPLE
DETECTING SYSTEM

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16
Q

ELECTROPHORESIS SEPERATES:

A

PROTEIN
PESTICIDE
DRUGS
AMINO ACIDS
NUCLEIC ACIDS

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17
Q

USES FILTER PAPER, ECONOMICAL

FOR LOW MW

A

PAPER ELECTROPHORESIS

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18
Q

PAPER ELECTRO SEPERATION TIME

A

12-14HRS

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19
Q

WHAT CANT BE RESOLVED BY PAPER E.

A

PROTEIN
HYDROPHILIC MOLECULES

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20
Q

TAKES - CHARGE FORM ADSORPTION OF HYDROXYL IONS

A

SUPPORT MEDIA

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21
Q

PAPER E. SUPPORT MEDIA: GREATER RESO, SOAKED IN WATER FILLED WITH ELECTROLYTES

A

CELLULOSE PAPER

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22
Q

CELLULOSE PAPER AIR SPACE

A

80%

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23
Q

PAPER E. SUPPORT MEDIA: USED AS PURIFIED FRACTION OF AGAR

NEUTRAL

A

AGAROSE GEL

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24
Q

MOVEMENT OF BUFFER ION AND SOLVENT RELATIVE TO FIXED SUPPORT

A

ELECTROENDOSMOSIS

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25
Q

AMOUNT NEEDED IN ELECTROENDOSMOSIS

26
Q

PAPER E. SUPPORT MEDIA: SEPERATES PROTEINS ON SURFACE CHARGE AND MOL SIZE

NOT WIDELY USED

27
Q

PAPER E. SUPPORT MEDIA: SEPERATION BASED ON CHARGE AND MOL SIZE

STUDY INDIV PROTEINS

A

POLYACRYLAMIDE GEL

28
Q

SEPERATION THROUGH MOLECULAR SIEVEING TECHNIQUE

A

GEL ELECTROPHORESIS

29
Q

PROTEINS ARE SEPERATED BASED ON CHARGE TO MASS RATIO AND MOL SIZE

A

MOLECULAR SIEVING PHENOMENON

30
Q

MOLECULAR SIEVING PHENOMENON BASIS (2)

A

CHARGE TO MASS RATIO
MOLECULAR SIZE

31
Q

E. GEL APARATUS: THICKNESS LIMITED, ONE GEL PER APARATUS

A

HORIZONTAL

32
Q

E. GEL APARATUS: DIFF THICKNESS, MORE THAN ONE GEL PER APARATUS

33
Q

ELECTROPHORESIS PROTEIN STAIN

A

AMIDO BLACK
BROMPHENOL BLUE
NIGROSIN

34
Q

ISOENZYMES USES WHAT STAIN

A

NITROTETRAZOLEUM BLUE

35
Q

PROTEIN STAIN DARK BLUE BAND

A

AMIDO BLACK

36
Q

PROTEIN STAIN GIVING RED BAND

A

PONCEAU S DYE

37
Q

LIPOPROTEIN STAINS

A

OIL RED O
SUDAN BLACK B

38
Q

MODIFICATION OF ELECTROPHORESIS

PROTEINS ARE SEPERATED BASED ON ISOELECTIC PH

A

ISOELECTRIC FOCUSING

39
Q

ISOELECTRIC FOCUSING MEDIA (3)

A

AGAROSE GEL
POLYACRILAMIDE GEL
CELLULOSE ACETATE

40
Q

ACIDIC GROUP > BASIC GROUP = LOW PH

A

ACIDIC PROTEIN

41
Q

BASIC > ACIDIC = HIGH ISOELECTRIC PH

A

BASIC PROTEIN

42
Q

ISOLECTRIC PH RANGE

43
Q

MIXTURE OF MOLECULES CONTAINING MULTIPLE ALIPHATIC AND AMINO CARBOXYLATE GROUPS

A

CARRIER AMPHOLYTES

44
Q

IEF MEANS

A

ISOELECTRIC FOCUSING

45
Q

DERIVATIVE OF ACRYLAMIDE (HAS DOUBLE BOND AND BUFFERING GROUPS )

A

ACRYLAMIDE BUFFER

46
Q

GEL FOR ISOELECTRIC FOCUSING POLYACRYLAMIDE IS POLYMERIZED WITH __

A

RIBOFLAVIN

47
Q

AUTOMATION ADVANTAGES (#)

A

RAPID RESULT
ECONOMICAL
ERRORS IN CALCULATIONS REDUC4ED

48
Q

1ST AUTOMATED ANALYZER YEAR

49
Q

FIRST ANALYZER BY

50
Q

BATCH ANALYZER IN 1957

A

CONTINOUS FLOW SINGLE CHANNEL

51
Q

CONTINOUS FLOW SINGLE CHANNEL CAN HANDLE __ SAMPLES/HOUR

52
Q

NEXT ANALYZER TO BE DEVELOPED

A

SIMULTANEOUS MULTIPLE ANALYZER

SMA 6, SMA 12

53
Q

SMA ___ RESULTS SIMULT AT RATE OF ___ PER HOUR

A

6-12, 360-720

54
Q

SPECIALTY AREA W RAPIDLY DEVELOPING ARSENAL OF ANALYZERS

A

IMMUNOCHEMISTRY

55
Q

LIQUIDS ARE PUMPED INTO SYSTEM OF CONTINOUS TUBING

A

CONTINOUS FLOW

56
Q

CONTINOUS FLOW SEPERATING AND CLEANING MEDIA

A

AIR BUBBLES AT REGULAR INTERVAL

57
Q

USES FORCE BY CENTRIFUGATION

A

CENTRIFUGAL ANALYSIS

58
Q

MOST POPULAR
- SEPERATION OF EACH SAMPLE INTO CONTAINER

  • CAN GO STAT
A

DISCRETE ANALYSIS

59
Q

WHEN ATOMS RETURN TO GROUND STATE, THEY EMIT RADIATION

A

FLAME EMISSION

60
Q

IF YOU PASS BY THIS, STUDY PICTURES, PASADAHAN AND PANGALANAN MO

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