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M-5 Flashcards

1
Q

-PERMITS AUTOMATIC CORRECTION OF SAMPLE/REFERENCE ABSORBANCE

A

DOUBLE BEAM SPECTROPHOTOMETER

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2
Q
  • NEEDED WHEN ABSORPTION SPECTRUM IS TO BE OBTAINED BECAUSE INTENSITIES OF LIGHT SOURCE VARY AS FUNCTION OF WAVELENGTH
A

DOUBLE BEAM SPECTROPHOTOMETER

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3
Q

DOUBLE SPECTRO: BEAMS OF LIGHT PASS THROUGH DIFFERENT COMPONENTS BUT AT THE SAME TIME IN THIS SPECTROPHOTOMETER

A

DOUBLE BEAM IN SPACE

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4
Q

IN DOUBLE BEAM IN SPACE ALL ARE DUPLICATED EXCEPT

A

LIGHT SOURCE

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5
Q

DOUBLE SPECTROPHOTOMETER: USES SAME COMPONENT AS SINGLE BEAM

A

DOUBLE BEAM IN TIME

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6
Q

DOUBLE BEAM IN TIME HAS DUPLICATE ___

A

CUVET COMPARTMENT

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7
Q

DOUBLE SPECTROPHOTOMETER: TWO BEAMS PASS THROUGH THE SAME COMPONENTS BUT NOT AT THE SAME TIME

A

DOUBLE BEAM IN TIME

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8
Q

DOUBLE SPECTROPHOTOMETER: LIGHT BEAM CHOPPER ROTATING WHEEL W/ ALTERNATE SILVERED SECTIONS

A

DOUBLE BEAM IN TIME

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9
Q

LESS EXPENSIVE SPECTROPHOTOMETER

A

SINGLE BEAM SPECTROPHOTOMETER

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10
Q

SPECTROPHOTOMETER: HAVE SIMPLE STRUCTURE

A

SINGLE BEAM

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11
Q

SPECTROPHOTOMETER: COMPLEX STRUCTURE

A

DOUBLE BEAM SPECTROPHOTOMETER

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12
Q

SPECTROPHOTOMETER: REQUIRES LESS COMPONENTS

CHANCES OF ERROR AS BLANK SOLUTION

ALL LIGHT WAVES COMING FROM LIGHT SOURCE PASSESS THROUGH SAMPLE

A

SINGLE BEAM SPECTROPHOTOMETER

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13
Q

SINGLE BEAM SPECTROPHOTOMETER REQUIRES

A

LIGHT SOURCE
SINGLE COMPARTMENT
CUVETTE
MONOCHROMATOR
DISPERSION DEVICVE
DETECTOR READ OUT DEVICE

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14
Q

SINGLE BEAM SPECTROPHOTOMETER USES____

A

NON SPLIT LIGHT BEAM

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15
Q

THIS SPECTROPHOTOMETER REQUIRES MORE COMPONENTS

A

DOUBLE BEAMSPECTROPHOTOMETER

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16
Q

DOUBLE BEAM ADDITIONAL COMPONENTS

A

MIRROR
CHOPPER
2 COMPARTMENT

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17
Q

SPECTROPHOTOMETER: BLANK AND SAMPLE ARE OPERATED AT THE SAME TIME

A

DOUBLE BEAM

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18
Q

SPECTROPHOTOMETER: LIGHT BEAM IS SPLIT INTO TWO FRACTIONS, REPRODUCIBLE

A

DOUBLE BEAM

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19
Q
  • LOW COST
  • HIGHER LIGHT THROUGHPUT
  • OPTICAL SYSTEM IS SIMPLE
A

SINGLE BEAM

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20
Q

-HIGH STABILITY
- ACCURATE
- FLUCTUATIONS DUE TO STRAY LIGHT
- CORRECTION OF ABSORBANCE

A

DOUBLE BEAM

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21
Q

SPECTROPHOTOMETER: COMPLICATED AND EXPENSEIVE

A

DOUBLE BEAM

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22
Q

SPECTROPHOTOMETER: CONTINOUS SPECTRUM NOT OBTAINED

A

SINGLE BEAM

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23
Q

IN SINGLE BEAM, FLUCTUATION IN INTENSITY OF RADIATION AFFECTS

A

ABSORBANCE

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24
Q

IN SINGLE BEAM, ABSORBANCE IS AFFECTED BY

A

ANY FLUCTUATION IN THE INTENSITY OF RADIATION

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25
Q

SPECTROPHOTOMETER: CALIBRATION WITH BLANK EVERYTIME

A

SINGLE BEAM

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26
Q

SPECTROPHOTOMETER: CALIBRATION ONLY IN BEGINNING

A

DOUBLE BEAM

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27
Q

SPECTROPHOTOMETER: RADIANT ENERGY CHANGES WITH VOLTAGE

A

SINGLE BEAM

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28
Q

SPECTROPHOTOMETER: LARGE DEGREES OF COMEPNSATION FOR FLUCTUATIONS

A

DOUBLE BEAM

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29
Q

SPECTROPHOTOMETER: MEASURES AMOUNT OF TRANSMITTED LIGHT REACHING

A

SINGLE BEAMM

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30
Q

SPECTROPHOTOMETER: MEASURES % OF LIGHT ABSORBED BY SAMPLE

A

DOUBLE BEAM

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31
Q

SPECTROPHOTOMETER: WHERE IS IT POSSIBLE TO COMPARE SAMPLE AND STANDARD

A

DOUBLE BEAM

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32
Q

SPECTROPHOTOMETER: WHERE THERE IS A NEED FOR RADIANT ENERGY ADJUSTMENT EVERYTIME

A

SINGLE BEAM

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33
Q

SPECTROPHOTOMETER: WHERE SCANNING CAN BE DONE OVER A WIDE WAVELENGTH

A

DOUBLE BEAM

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34
Q

SPECTROPHOTOMETER: WHICH IS TEDIOUS AND TIME CONSUMING

A

SINGLE BEAM

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35
Q

ELECTRONS ARE EXCITED IN FLAME AND EMIT RADIATION OF A WAVELENGTH WHEN RETURNING TO GROUND STATE

A

FLAME PHOTOMETRY

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36
Q

FLAME PHOTOMETRY METHOD

A

INDIRECT INTERNAL STANDARD

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37
Q

PARTS OF FLAME PHOTOMETRY

A

ATOMIZER
FLAME
AIR&GAS SUPPLY
MONOCHROMATOR
DETECTOR
READOUT DEVICE

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38
Q

FLAME PHOTOMETRY USED ELEEMENTS

A

NA, K, LI

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39
Q

FLAME PHOTOMETRY: NA, K

A

NA -YELLOW
K - VIOLET

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40
Q

FLAME PHOTOMETRY: LI, RB, MG

A

LI - RED
RB - RED
MG - BLUE

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41
Q

TEMP: 1700

A

NATURAL GAS AIR

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42
Q

TEMP: 1800

A

PROPANE AIR

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43
Q

TEMP: 2000

A

HYDROGEN-AIR

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44
Q

TEMP: 2650

A

H-O

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45
Q

200

A

ACETYLENE OXYGEN

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45
Q

TEMP: 2300

A

ACETYLENE-AIR

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46
Q

TEMP: 2700

A

ACETYLENE-NITROUS OXIDE

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47
Q

TEMP: 4800

A

CYONOGEN-OXYGEN

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48
Q

FLAME PHOTOMETER GASES

A

MIX OF HYDROGEN + GAS
- ACETYLENE
- PROPANE
- NATURAL GAS

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49
Q

DRAWS SAMPLE INTO FLAME

A

ASPIRATOR

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50
Q

CREATES FINE SPRAY OF SAMPLE SOLUTION

51
Q

BREAKS CHEMICAL BONDS/EXCITES ELECTRONS

52
Q

GASES ARE PASSED AT HIGH VELOCITY–LIQUID DRAWN UP THROUGH CAPILLARY

A

TOTAL CONSUMPTION BURNER

53
Q

GRAVITATIONAL FEEDING OF SOLUTION THROUGH RESTRICTING CAPILLARY

A

LAMINAR FLOW BURNER

54
Q

IN LAMINAR FLOW BURNER HOW MANY LOSS OF GAS

55
Q

NARROWEST BANDPATH MAXIMUM AMOUNT OF LINE EMISSION TO PASS THROUGH DETECTOR

A

MONOCHROMATOR

56
Q

WHAT DOES FLAME SPECTROPHOTOMETRY USE AS DETECTOR

57
Q

FLAME SPECTRO INTERNAL STANDARD (2)

A

LITHIUM OR CESIUM

58
Q

FLAME COLOR AND EMISSION WAVELENGTH: BARIUM

A

554, LIME GREEN

59
Q

FLAME COLOR AND EMISSION WAVELENGTH: SODIUM

A

589, YELLOW

60
Q

FLAME COLOR AND EMISSION WAVELENGTH: CALCIUM

A

662, ORANGE

61
Q

FLAME COLOR AND EMISSION WAVELENGTH: LITHIUM

62
Q

FLAME COLOR AND EMISSION WAVELENGTH: POTASSIUM

A

766, VIOOLET

63
Q

CORRECTS FLUCTUATION IN AIR AND GAS PRESURE

A

INTERNAL STANDARD

64
Q

MEASURES LIGHT ABSORBED BY GROUND STATE ATOMS

A

ATOMIC ABSORPTION SPECTROPHOTOMETRY

65
Q

FLAME SOURCE OF AAS

A

HOLLOW CATHODE LAMP

66
Q

MEASURE UNEXCITED TRACE METALS

A

ATOMIC ABSORPTION SPECTROPHOTOMETRY

67
Q

UNEXCITED TRACE METALS

A

CA, MG, CU, ZN, PB

68
Q

WHICH MEASURES
RADIANT
THERMAL

A

R - FEP
T - AAS

69
Q

WHICH MEASURES
LIGHT EMMISION
LIGHT ABSORBANCE

A

LE - FEP
LA - AAS

70
Q

FAILURE OF FLAME TO DISSOCIATE THE SAMPLE INTO FREE ATOMS

A

CHEMICAL INTERFERENCE

71
Q

ADDED TO FORM STABLE COMPLEXES WITH PHOSPHATE

A

LANTHANUM AND STRONTIUM

72
Q

EXCITATION INSTEAD OF DISSOCIATION

A

IONIZATION INTERFERENCE

73
Q

LIGHT IS ABSORBED BY ORGANIC SOLVENTS

A

MATRIX INTERFERENCE

74
Q

CAUSED BY FORMATION SOLDS FROM SAMPLE DROPLETS

A

LIGHT ABSORPTION

75
Q

LIGHT SOURCE OF AAS

A

HOLLOW CATHODE LAMP

76
Q

MODULATES LIGHT BEAM

77
Q

USES FLAME TO DISSOCIATE

78
Q

SELECTS DESIRED WAVELENGTH

A

MONOCHROMATOR

79
Q

MEASURES INTENSITY OF LIGHT SIGNAL

80
Q

DETECTOR USES

A

PHOTOMULTIPLIER TUBES

81
Q

AAS BURNER: FLAME IS MORE CONCENTRATED Q

A

TOTAL CONSUMPTION BURNER

82
Q

AAS BURNER: GASES ARE MIXED AND SAMPLE IS ATOMIZED

A

PREMIX BURNER

83
Q

AAS BURNER: HEATED UNTIL CHARRED

A

FLAMELESS AAS

84
Q

FLAMELESS AAS USES

A

CARBON ROD OR GRAPHITE FURNACE

85
Q

ATOMS ABSORB LIGHT OF A WAVELENGTH AND EMIT LONGER WAVELENGTH

A

FLUORESENCE SPECTROPHOTOMETRY

86
Q

FLUORESENCE SPECTROMETRY ENERGY SOURCE

A

MERCURY LAMP
XENON LAMP

87
Q

ENERGY EMISSION THAT OCCURS WHEN CERTAIN COMPOUND ABSORB EM

A

FLUORESENCE

88
Q

FS LIGHT SOURCE

A

HYDROGEN DISCHARGE LAMP
XENON LAMP

89
Q

ISOLATES UV LIGHT IN FS

A

PRIMARY/ EXCITATION FILTER

90
Q

ISOLATES SECONDARY EMISSION

A

SECONDARY / EMISSION FILTER

91
Q

MOST PREFERED EMISSION FILTER

A

DIFFRACTION GRATING

92
Q

MEASUREMENT OF LIGHT BLOCKED BY SUSPENSION

A

TURBIDIMETRY

93
Q

FACTORS AFFECTING TURBIDIMETRY

A

SIZE AND NUMBER OF PARTICLES
TUBE DEPTH
CROSS SECTIONAL AREA OF EACH PARTICLE

94
Q

MEASUREMENT OF LIGHT SCATTERED BY PARTICULATE MATTER SUSPENDED IN A TURBID SOLUTION

A

NEPHELOMETRY

95
Q

NEPHELOMETRY ANGLE

A

15-90 DEGREES

96
Q

MEASURES DEFLECTION OF LIGHT RAY FROM STRAIGHT LINE

A

REFRACTOMETRY

97
Q

ABILITY TO BEND LIGHT

A

REFRACTIVITY

98
Q

DETERMINES OSMOLALITY BASED ON FREEZING POINT DEPRESSION

99
Q

MOST COMMONLY USED METHOD FOR MEASURING CHANGES IN COLLIGATIVE PROPERTIES

A

FREEZING POINT DEPRESSION OSMOMETRY

100
Q

COLLIGATIVE PROPERTIES OF A SOLUTION

A

OSMOTIC PRESSURE
BOILING P.
FREEZING P.
VAPOR PRESSURE

101
Q

PROPERTIES THAT DEPEND ON CONCENTRATION ON SOLUTE MOLECULE S

A

COLLIGATIVE PROPERTIES

102
Q

OSMOTIC PARTICLES

A

GLUCOSE
UREA
NITROGEN
SODIUM

103
Q

USED TO MEASURE DISINTEGRATION OF RADIOISOTOPE PER MINUTE

A

SCINTILLATION COUNTER

104
Q

CHEMICALS USED TO CONVERT THEIR ENERGY INTO LIGHT

A

SCINTILLATORS

105
Q

+, RESEMBLE NUCLEUS OF HELIUM ATOM (MW:4)

A

ALPHA RADIATION

106
Q

RESEMBLES ELECTRON WITH BOTH -, + CHARGE BUT NO MASS

A

BETA RADIATION

107
Q

EM ENERGY WITH NO MASS, ONLY ENERGY

A

GAMMA RADIATION

108
Q

PHOTOM THAT CARRIES ENERGY IN FORM OF EM WAVES

A

GAMMA PARTICLES

109
Q

MEASURES GAMMA RADIATION

A

SOLID SCINTILLATION COUNTER

110
Q

SOLID SCINTILLATION COUNTER USES __ TO MEASURE

A

THALLIUM ACTIVATED SODIUM IODIDE CRYSTAL

111
Q

MEASURES BETA RADIATION

A

LIQUID SCINTILLATION COUNTER

112
Q

LIQUID SCINTILLATION COUNTER USES?

A

LIQUID FLUOR

113
Q

AROMATIC LIQUIDS/MIXTURE OF LIQUIDS

114
Q

EMITS 200-300NM

A

PRIMARY FLUOR

115
Q

ABSORBS LIGHT AT 200-300NM

A

SECONDARY FLUOR

116
Q

IMMUNOLOGIC PROCEDURE INVOLVING USE OF RADIOISOTOPE

MEASURE CONC OF ANTIGEN

A

RADIOIMMUNOASSAY

117
Q

RIA SUBSTANCE: SUNSTANCE BEING ANALYZED

A

UNLABELLED ANTIGEN

118
Q

RIA SUBSTANCE: ACTS AS LABEL

A

RADIOLABELLED ANTIGEN

119
Q

PROVIDE BINDING SITE FOR 2 ANTIGEN

120
Q

ANTIBODY VS ANTIGEN

A

ANTIBODY - IN THE BODY
ANTIGEN - FOREIGN

121
Q

MEASUREMENT OF AMOUNT OF ELECTRICITY AT FIXED POTENTIAL

A

COULOMETRY

122
Q

ELECTROCHEMICAL TITRATION WHICH TITRANT IS ELECTROCHEMICALLY GENERATED

A

COULOMETRY

123
Q

COULOMETRY MEASURED IN

124
Q

COULOMETRY FOLLOWS ___

A

FARADAY’S LAW

125
Q

COULOMETRY INTERFERENCES (3)

A

BROMIDE
CYANIDE
CYSTEINE

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