M1&2 Flashcards

1
Q

List characteristics of Prokaryotes

A
  • Small and primitive
  • All bacteria
  • No membrane bound organelles but has ribosomes
  • No nucleus, but has nucleoid
  • Cell wall with peptidoglycan
  • One circular chromosome
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2
Q

List characteristics of Eukaryotes

A
  • Large and complex
  • All other organisms
  • Membrane-bound organelles
  • Nucleus and nuclear membrane
  • No cell wall, but has cell membrane
  • Many chromosomes, not circular
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3
Q

How is bacteria classified?

A
  • Structure and shape: Cell wall, Antigens, Morphology
  • Metabolic processes: Aerobic / anaerobic / facultative, Fermentation of sugars
  • Other biochemical reactions / production of specific enzymes or toxins
  • DNA sequence
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4
Q

Is a baterium a prokaryote or eukaryote?

A

prokaryote

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5
Q

Is a fungus a prokaryote or eukaryote?

A

eukaryote

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6
Q

How is a fungus classified?

A

It has its own kingdom

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7
Q

What are the 3 different types of fungi?

A

yeasts, mould, dimorphic (yeast/mould depending on temperature)

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8
Q

What is distinctive of fungi?

A
  • distinct cell membrane: ergosterol

- distinct cell wall

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9
Q

What is the difference between yeasts and moulds in terms of how long they take to grow?

A
  • Yeasts: 2-3 days

- Moulds: days-weeks

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10
Q

What is the difference between yeasts and moulds in terms of temperature?

A
  • Yeasts: grow best at 35°C

- Moulds: variable temperatures (often 25°C)

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11
Q

What is the difference between yeasts and moulds in terms of colonies?

A
  • Yeasts: colonies look like bacteria

- Moulds: “fluffy colonies”

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12
Q

What are examples of yeasts?

A

Candida spp, Cryptococcus

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13
Q

What are examples of moulds?

A

Aspergillus, Penicillium

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14
Q

Define parasite

A

An organism that lives on or in a host and gets its food from or at the expense of its host, not a specific class of organism.

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15
Q

List characteristics of protozoa

A

Eukaryotic
Unicellular
Multiple classes

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16
Q

What are examples of protozoa?

A

Malaria, Trichomonas, Giardia, amoebae

17
Q

Name characteristics of metazoa

A
  • Trematodes, nematodes, cestodes etc.
  • Eukaryotic
  • Multicellular
  • Multiple classes
18
Q

What are examples of metazoa?

A

Hookworm, whipworm, bilharzia

19
Q

Name important facts about bacteria nomenclature

A
  • Genus and species usually tells you something about the organism, the disease it causes or the person who discovered it.
  • Often derived from Latin or Greek
  • Either underlined or written in italics.
  • Only the first letter of the genus is written in uppercase, and you may use this first letter as an abbreviation (eg. E. coli).
20
Q

In the example: Entamoeba coli, which one is the genus name and which one is the species name?

A
  • Entamoeba: genus name

- coli: species name

21
Q

What are the 4 groups of bacteria?

A
  • Mycobacteria
  • Gram positive
  • Gram negative
  • Atypical bacteria
22
Q

What is the structure of Gram positive bacteria?

A
  • Stain purple
  • Thick peptidoglycan layer with no outer membrane
  • Cross-linking between peptidoglycan molecules: some antibiotics target cross-link sites
23
Q

What is the structure of Gram negative bacteria?

A
  • Stains pink
  • Thin peptidoglycan layer with outer membrane
  • Contains lipopolysaccharides: endotoxins in endotoxic shock
  • Periplasmic space contains β-lactamase enzymes
24
Q

Describe the structure of mycobacteria

A

Rod- shaped organisms with complex cell wall, acid-fast stain

25
What are the different types of atypical (no/incomplete cell wall) bacteria?
Rickettsia, Chlamydia, Spirochaetes, Mycoplasma
26
What is the structure of Rickettsia and chlamydia?
Intracellular organisms, Cell wall thin, Don’t stain with Gram stain due to size and location
27
What is the structure of Spirochaetes?
Very thin organisms, and don’t stain with Gram stain
28
What is the structure of mycoplasma?
No cell wall, so don’t stain with Gram stain