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Fundamentals of Biochemistry > M05 Protein Studies > Flashcards

M05 Protein Studies Flashcards

1
Q

What are the general steps of extracting cellular proteins?

A
  1. Cell rupture
  2. Differential centrifugation
  3. Purify soluble protein
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2
Q

What is the purpose cell rupture?

A
  • Release proteins from cells and organelles
  • Homogenization used to break open cells
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3
Q

What are the basic principles of differential centrifugation?

A
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4
Q

What is the general order of precipitation in differential centrifugation?

A
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5
Q

What are the general principles of salt fractionation (salting out)?

A
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6
Q

What does the presence of salt accomplish in salt fractionation?

A
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7
Q

What is the purpose of salt fractionation?

A
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8
Q

What is the general order of protein purification steps?

A
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9
Q

What is total activity?

A
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10
Q

What is specific activity?

A
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11
Q

How does total protein change with each step in purification?

A
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12
Q

How does specific activity change with each step in purification?

A
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13
Q

What is chromatography?

A
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14
Q

How can primary structure of proteins be determined?

A
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15
Q

What are the two phases of chromatography?

A
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16
Q

What are the protein detection techniques?

A
17
Q

What is the purpose of the stationary phase in chromatography?

A
18
Q

What is the purpose of the mobile phase in chromatography?

A
19
Q

What is the method of column chromatography?

A
20
Q

What is the method of size exclusion (gel filtration) chromatography?

A
21
Q

How do particles separate based on size in size exclusion chromatography?

A
22
Q

What are the principles of affinity chromatography?

A
23
Q

What is ion exchange chromatography?

A
24
Q

What is the difference between anion and cation exchangers?

A

Fundamentals of Biochemistry (5 decks)

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