M05 Protein Studies Flashcards
What are the general steps of extracting cellular proteins?
- Cell rupture
- Differential centrifugation
- Purify soluble protein
What is the purpose cell rupture?
- Release proteins from cells and organelles
- Homogenization used to break open cells
What are the basic principles of differential centrifugation?
What is the general order of precipitation in differential centrifugation?
What are the general principles of salt fractionation (salting out)?
What does the presence of salt accomplish in salt fractionation?
What is the purpose of salt fractionation?
What is the general order of protein purification steps?
What is total activity?
What is specific activity?
How does total protein change with each step in purification?
How does specific activity change with each step in purification?
What is chromatography?
How can primary structure of proteins be determined?
What are the two phases of chromatography?
What are the protein detection techniques?
What is the purpose of the stationary phase in chromatography?
What is the purpose of the mobile phase in chromatography?
What is the method of column chromatography?
What is the method of size exclusion (gel filtration) chromatography?
How do particles separate based on size in size exclusion chromatography?
What are the principles of affinity chromatography?
What is ion exchange chromatography?
What is the difference between anion and cation exchangers?
