Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

OD2 S1 > M: Virology 4 - Week 12 > Flashcards

M: Virology 4 - Week 12 Flashcards

1
Q

What must a virus do to persist? (2)

A

Maintain (but control expression of) its genome
Avoid host defence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Is HIV a fast or slow infection?

A

HIV is a slow persistent infection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

How many deaths has HIV being responsible for from its beginning until 2012?

A

42.5 million deaths

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

How many people in 2012 were estimated to be living with HIV and AIDS?

A

35.3 million

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

How many people have been cured of HIV?

A

2 people. A Berlin patient (via stem cell transplant) and a London patient

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How many people currently living in Australia have been diagnosed with HIV?

A

23,100

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What proportion of living Australians diagnosed with HIV are:
A: People who inject drugs
B: Sex workers
C: Aboriginal/Tores Straight

A

A: People who inject drugs - 1-2%
B: Sex workers - less than 0.1%
C: Aboriginal/Tores Straight - 0.15% (however this one is increasing dramatically)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the most common transmission method for HIV in Australia?

A

male homosexual contact

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the most common transmission method for HIV in the world?

A

Heterosexual contact

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

In regard to HIV:
A: What family of virus does it belong to?
B: How many types? Name them
C: What is its capsid symmetry?

A

A: Retroviridae
B: 2 types. HIV-1 and HIV-2
C: Icosahedral symmetry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

In regard to HIV:
D: Describe its genome (i.e. DNA or RNA? Positive or Negative sense? Size? etc.)

A

Diploid linear 9.2kb positive-sense ssRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

In regard to HIV:
E: Does it have an envelope?
F: Where is its genome replicated?

A

E: Yes
F: Nucleus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

In regard to HIV:
G: Where doe HIV virus assembly occur?

A

plasma membrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is virus assembly?

A

During the replication of many viruses, hundreds to thousands of proteins assemble around the viral nucleic acid to form a protein shell called a capsid.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

In regard to HIV:
H: What diseases can it commonly cause? (4)

A

AIDS
Neurologic
Arthritis
Pneumonia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

In regard to HIV:
I: What is its origin?

A

Zoonosis from chimpanzees

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

HIV is a “lentivirus”. What does this mean?

A

Lentivirus = any of a group of retroviruses producing illnesses characterized by a delay in the onset of symptoms after infection.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What does AIDS stand for?

A

Acquired Immune Deficiency Syndrome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

In regard to HIV structure:
- what are the 3 different layers in the HIV virus?

A

Inner layer
Protein layer (middle layer) (called the “matrix”)
Envelope layer (outer layer)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What is found in the inner layer of HIV? (6)

A

Enzymes: Reverse Transcriptase (RT), Integrase (IN), Protease (PR)

Also: Capsid (is the outer boundary) Nucleocapsid, and 2 +ve sense ssRNA strands

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What are the enzymes found in the inner layer of HIV encoded by?

A

pol gene

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What is found in the protein layer of HIV? (1)

A

Matrix protein

(also some enzymes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Describe the inner layer of HIV.

A

The inner layer is formed by the capsid (protein shell), which is the outer membrane of the virion’s nucleus. Within the capsid you have the nucleocapsid, 2 viral +ve sense ssRNA strands, and the enzymes RT, IN and PR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

What encodes the protein layer of HIV?

A

Gag gene

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

What is found in the envelope layer of HIV? (2)

A

gp120 (surface cell attachment protein)
gp41 (transmembrane domain)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

How variable are gag and envelope proteins?

A

Highly variable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

What does the Gag gene encode? (4)

A

Structural proteins of:
- the capsid
- the matrix
- the core
- the nucleocapsid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

What does the pol gene encode? (4)

A

viral enzymes: protease, reverse transcriptase, RNase H and Integrase enzymes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

What are envelope glycoproteins expressed from?

A

a spliced mRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

What shape is the capsid of HIV?

A

cone-shaped

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

What is the role of protease enzymes? (in HIV)

A

They chop the long polyproteins into individual units

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Describe the HIV replication cycle, excluding exit from the cell (5 steps)

A
  1. Envelope surface glycoprotein gp120 binds CD4 molecule expressed on the surface of the immune cell
  2. During this, a co-receptor is bound
  3. These bindings result in the release of RNA genome into the cytoplasm of the cell
  4. HIV’s RT converts the RNA genome into a double-stranded DNA copy, which gets integrated into the host cell
  5. Host cell replicates the viral DNA
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Describe the HIV replication cycle: Once the virus replicates, how does the viral DNA leave? (5 steps)

A
  1. In addition to being the source of new viral RNA copies, the Integrated proviral DNA acts as mRNA
  2. This “mRNA”: produces gag and pol polyproteins
  3. This “mRNA”: undergoes splicing to produce envelope RNA + envelope glycoproteins
  4. these contents assemble at the plasma membrane of the infected cell
  5. “Budding” occurs: during this, the polyprotein is cleaved by protease and packaged up inside the new virion
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

What happens to the to-be-released virion if protease is not present?

A

The polyprotein is not cleaved and packaged inside the new virion. The released virion is now considered: NOT INFECTIOUS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

What parts of the HIV replication cycle can we target for antiretroviral therapy? (4)

A

Fusion/Entry inhibitors
Reverse Transcriptase inhibitors
Integrase inhibitors
Protease inhibitors

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

What co-receptor is typically bound during HIV viral entry into a cell?

A

CCR5

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

What do integrase inhibitors do (in relation to HIV)?

A

Prevent the viral enzyme from stitching the copy of DNA into the target cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

Why is cleavage of the polyprotein required for the virion to be infectious?

A

Cleavage is required to form active RT and active integrase, which are required to make the virion infectious

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

What immune cell does HIV target?

A

CD4 T cells (Helper-T cells)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

What is the role of CD4+ “helper” T cells? (4)

A

Lead and coordinate the immune system’s response to infection:
- “help” B cells make antibody
- “help” macrophages kill bacteria
- “help” killer T cells destroy viruses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

Are CD4+ “helper” T cells involved in memory?

A

Yes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

What are the subgroups of CD4+ T cells (i.e. what kinds of T cells do/can express CD4)?

A

TH1
TH2
Tregs
LN (T-follicular helper cells)
Naive or Memory
Resting or activated

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

What is the role of LN (T-follicular helper cells)?

A

Instruct B cells in the germinal centre to make effective antibody responses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

What are the 2 cell surface receptors for HIV?

A

CD4
Chemokine receptors

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

What 2 chemokine receptors can act as co-receptors for HIV? Which one is more prominently used in HIV transmission?

A

CCR5 - the predominant one
CXCR4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

In what notable circumstance might CXCR4 be used as a co-receptor for the HIV virus?

A

When there is no CCR5 present (i.e. if either the cell doesn’t have CCR5 to begin with, or if the CCR5 was removed somehow)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

What 2 cell types have both chemokine receptors available to act as co-receptors for HIV?

A

CD4 T lymphocytes
Certain macrophages

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

What is the function of HIV reverse transcriptase?

A

converts the viral genomic RNA to proviral cDNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

How accurate is the action of HIV’s reverse transcriptase?

A

Poor. High error rate (error rate = 1:10,000nt)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
50
Q

Which drug target is the most important for HIV?

A

RT (Reverse Transcriptase)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
51
Q

What is the function of HIV’s integrase?

A

catalyses the random integration of HIV cDNA into cell DNA

52
Q

What is required for the production of a new HIV virus?

A

Integrase

53
Q

In what cell states can integration occur in? (2)

A

resting and terminally differentiated cells

54
Q

How does reverse transcriptase duplicate the sequences in HIV?

A

duplicates the sequences at the ends of the viral RNA forming a DNA structure called the “long terminal repeat” or LTR

55
Q

What is the function of the 5’ region of the LTR (long terminal repeat) in HIV?

A

acts as the HIV gene promoter

56
Q

How does 5’-LTR respond to the tat protein in HIV?

A

greatly increases in expression

57
Q

How does 5’-LTR affect HIV expression after initial replication?

A

silences HIV expression after initial replication

58
Q

Does 5’-LTR respond to cellular proteins? Explain. What is the outcome?

A

Responds to cellular proteins made during T-cell immune activation to dramatically increase HIV expression

59
Q

Name 2 proteins essential for HIV replication

A

Tat and Rev proteins

(there are others obv., but this was on the particular slide)

60
Q

Name 4 proteins NOT essential for HIV replication in vitro.

A

Vif
Vpr
Vpu
Nef

61
Q

What is the role of tat (protein)?

A

transactivator of HIV transcription through TAR RNA

(Note: transactivation is the increased rate of gene expression triggered either by biological processes or by artificial means, through the expression of an intermediate transactivator protein.)

62
Q

What is the role of Rev (protein)?

A

regulator of structural gene expression through binding of the Rev-responsive RNA element (RRE)

63
Q

What is the role of Vif (protein)?

A

“viral infectivity factor”. Assists particle assembly and maturation, protects cDNA during reverse transcription

64
Q

What is the role of Nef (protein)?

A

Multifunctional protein important for in vivo pathogenesis. Down-modulates cell MHC-1 and CD4

65
Q

What is the role of Vpr (protein)?

A

virion protein for nuclear import of preintegration complex cell growth arrest, and weak transcription trans-activation

66
Q

What is the role of Vpu (protein)?

A

regulator of particle release and env. processing. Promotes MHC-1 and CD4 degradation

67
Q

Name 6 proteins essential for in vivo pathogenesis of HIV

A

Tat
Rev
Vif
Vpr
Vpu
Nef

68
Q

Which HIV drugs act DIRECTLY against the HIV’s ability to replicate? (3)

A

RT inhibitors
Protease inhibitors
Integrase inhibitors

69
Q

What happens to the icosahedral core of HIV after budding?

A

matures to form a complex rod shape

70
Q

What is an acute HIV infection characterised by?

A

a rapid and massive loss of the body’s CD4+ T cells

71
Q

Describe the timeline of HIV infection (5)

A

Primary infection, followed by a period of clinical latency that can last years (e.g. 7 years). During this period, the number of blood CD4+ T cell decreases until reaching a critical threshold, upon which constitutional symptoms occur, opportunistic diseases occur and eventually death.

72
Q

What is the critical threshold for HIV symptoms?

A

Around 200 CD4+ T cells per ml of blood

73
Q

What 2 things happen at the start of a HIV infection? (Just after the start of the primary infection)

A

Wide dissemination of virus
Seeding of lymphoid organs

74
Q

What happens to the level of body (not blood) CD4+ T cells during the primary infection stage of HIV?

A

Decreases dramatically

75
Q

What symptoms can occur in primary HIV infection? (12)

A

Fever
Myalgia/arthralgia
Nausea/vomiting/diarrhea
Weight loss
Malaise/lethargy
Rash
Lymphadenopathy
Pharyngitis
Oral thrush
Headache/retro-orbital pain
Meningitis
Transient CD4 depletion

*so basically everything honestly. What a long list. That last one seems the most important

76
Q

Describe the timeline of HIV that ends in widespread dissemination of the virus (7)

A
  1. Mucosal exposure to HIV-1
  2. Selective infection by R5 strains
  3. HIV binds to dendritic cell by DC-SIGN
  4. Transport of virus to regional lymph nodes
  5. Spread of infection to activated CD4+ T lymphocytes
  6. Entry of virus infected cells into bloodstream
  7. Widespread dissemination
77
Q

What is DC-SIGN?

A

Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin)

is a C-type lectin receptor present on the surface of both macrophages and dendritic cells.

78
Q

(HIV dissemination): Once bound to the dendritic cell, where does HIV get transported to?

A

regional lymph nodes (where the infection spreads to activated CD4+ T lymphocytes)

79
Q

What happens following transmission of a single R5-tropic HIV from the swarm?

A

Rapid evolution

80
Q

When is the window of opportunity for HIV vaccines?

A

Acute/Early phase of HIV dissemination (or before it, you know)

81
Q

Is humoral immunity present during acute/early HIV dissemination?

A

No it is not.

82
Q

In what phase of HIV dissemination is T-cell immunity and humoral immunity present?

A

seroconversion

83
Q

In what phase of HIV dissemination do HIV tests become positive for HIV?

A

When seroconversion occurs (usually within a few weeks of infection), the result of an HIV antibody test changes from HIV negative to HIV positive.

84
Q

In regard to HIV, what is seroconversion?

A

seroconversion is the time period during which a specific antibody develops and becomes detectable in the blood

85
Q

Which phase of HIV is considered the “fast phase”?

A

The primary infection

86
Q

What effect does HIV have on mucosal T-cells initially?

A

HIV infects and kills over 60% of mucosal T-cells within days (both activating and resting CD4+ CCR5+ memory T cells)

87
Q

What are the pathological effects of initial/primary HIV-1 infection? (3)

A

Kills over 60% of mucosal T cells within days
Massive loss of memory T cells in GALT
Initial decline in HIV due to CD4 substrate exhaustion

88
Q

How does the immune system respond to HIV? (2)

A

Initially contains the HIV replication, but then a virological set point is established:
- i.e. an equilibrium between viral replicative capacity and host anti-HIV defence

89
Q

How is the virological set-point in HIV measured? (1) What is considered “undetectable” in this test? (1)

A

RT-PCR for viral RNA form virus in blood
- undetectable viral load = less than 50 copies of viral RNA per ml

90
Q

What influences the HIV virological set-point from person to person?

A

determined by each person’s HLA type

91
Q

How does HIV mainly escape from the immune response?

A

escapes due to the extremely high viral mutation rate from reverse transcriptase errors

92
Q

By what mechanisms can HIV escape from the immune response? (5)

A

Mutation in viral epitopes that comprise recognition by cytotoxic T-lymphocytes
Viral mutations affecting binding to MHC or TCR
Clonal deletion of HIV-specific CD4+ T cells
Inhibition of antigen processing and/or presentation
Other mechanisms

93
Q

What does HIV viral mutation lead to? (3)

A

T cell anergy
peptide antagonism

94
Q

What does HIV escaping from the immune response lead to? (1)

A

Ongoing damage and depletion of CD4+ T cells

95
Q

What causes the T cell depletion in HIV? (2)

A

CD4+ T cell destruction
Chronic immune-activation

96
Q

How can CD4+ T cell destruction occur? (2)

A

Direct destruction of infected cells
Indirect destruction of uninfected cells

97
Q

How can indirect destruction of uninfected T cells occur in HIV? (3)

A

Cytolisis by HIV-specific CTLs or NK cells
Incorporation into syncitia (fused cells)
Immune activation of CD4 and CD8 T cells

98
Q

What are the pathological effects of chronic immune-activation in HIV? (7)

A

Loss of gut mucosal barrier integrity
Leakage of microbial products into systemic circulation
Stimulation of immune cells via PRR (TLR) activation
Elevation of pro-inflammatory cytokines
Death of CD4+ T cells
CD8+ T cells get trapped in lymph nodes
B-cell make auto-Antibody

99
Q

If chronic immune-activation in HIV was “The Trapper” from Dead by Daylight: What would get trapped, and where?

A

CD8+ T cells get caught in The Trappers trap. This occurs in the lymph nodes

CD4 cells can’t come to save them because they already died. (but they weren’t trapped, they just died)

100
Q

What happens to the immune system as a result of immune activation against HIV? (4)

A

Immune cell depletion
Immune cell dysfunction
Aberrant lymphocyte turnover (so lots of these cells die off)
Organ system dysfunction (e.g. cardiovascular disease)

101
Q

What effects does HIV have on T cells without therapy? (2)

A

Reduced T help for B cells and macrophages
Reduced CTL capacity to counter other viruses

102
Q

Is HIV the main cause of death for individuals who have it?

A

No. Most often its the associated infections that kill the patients

103
Q

Name 10 AIDS related illnesses (10)

A

Hairy leukoplakia (*Hairy Luke: someone named Luke, who is hairy)
HIV assoc. gingival destruction
dermatitis on eye via HSV
Primary CNS lymphoma via EBV
Cytomeglavorius retinopathy
Pneumocystis jirovecii Pneumonia (PCP)
Oral infections with candida albicans
Kaposi’s sarcoma
Cachexia, wasting
Lipodystrophy

104
Q

What is “Hairy Luke”? lol.

A

Hairy leukoplakia: caused by reactivation of the EBV herpesvirus

105
Q

Name 3 AIDS related illnesses that are caused by reactivation of latent viral infections

A

HSV virus (dermatitis on eye)
Epstein Barr virus (EBV. Primary CNS lymphoma)
Cytomegalovirus in the ey

106
Q

What can you use to treat dermatitis on the eye caused by HSV? (2)

A

acyclovir or valacyclovir

107
Q

What can you use to treat retinopathy caused by cytomegalovirus (CMV)? (3)

A

acyclovir, gancyclovir or valacyclovir

108
Q

What virus is responsible for Kaposi’s sarcoma?

A

human herpesvirus 8 (HHV-8)

109
Q

Name 2 types of kaposi’s sarcoma

A

oral and facial

110
Q

In relation to AIDS, how does lipodystrophy occur?

A

toxicity from treatment

111
Q

When is HIV active?

A

at all times, even when patient feels fine

112
Q

What measures can we take to control the HIV epidemic? (6)

A

Education
Testing
Condoms (?pregnancy?)
Circumcision
Cheap effective drugs
Cheap effective vaccine

113
Q

How does circumcision help to reduce the risk of acquiring HIV?

A

it removes the CCR5 receptors on the dendritic cells on the foreskin, thus reducing transmission

114
Q

Name 3 cheap effective drugs used to control HIV

A

Nucleoside analogues
Non-nucleoside analogues
Protease inhibitors

115
Q

Name 6 drug options for antiretroviral therapy for HIV. Do we use these in combination?

A

NRTI
NNRTI
Protease inhibitors
Fusion inhibitors
Integrase inhibitor
CCR5 antagonist

At least 3 drugs in combination

116
Q

What is the rationale for combination therapy (i.e. HAART)? (3)

A

Potency
Durable antiviral response
Minimise development of drug resistance (it is unlikely that multiple different changes would occur to form resistance against multiple drugs)

117
Q

Why were early HAART regimes difficult for patients/had difficulty with adverse effects and compliance?

A

They had to take 25 pills per day

118
Q

What were the major limitations of early HAART?

A

Pill burden and side effecs
Long term complications: Lipoatrophy, lipodystrophy, neuropathy
Emergence of multi-drug resistant HIV

119
Q

Imagine this scenario: It’s 1998. After watching mankind fall off hell in a cell, plummeting 16 feet towards an announcer’s table: you notice somebody standing next to you has a yellowing/jaundice of their face and they are chugging pills like no tomorrow. Assuming they aren’t a drug addict, what is likely wrong with this person?

A

They have HIV and are on early HAART therapy.

120
Q

How many pills does modern HAART therapy involve?

A

1 pill per day. Massive improvement!!

(incidentally it’s been about since 2010 that we’ve moved to 1 pill a day, based on my research)

121
Q

Compare the life expectancy of modern HAART patients against healthy control?

A

Still 10 years shorter (but better than early HAART)

122
Q

What is the half-life of HIV?

A

About 1 day

123
Q

What effects does HIV have on neutrophils without therapy? (1)

A

reduced killing of bacteria

124
Q

What effects does HIV have on B cells without therapy? (4)

A

general increase in antibodies
autoantibody formation
poor response to vaccines
reduced killing of encapsulated bacteria

125
Q

What effects does HIV have on macrophages without therapy? (3)

A

reduced phagocytosis
reduced chemotaxis
reduced killing

126
Q

What effects does HIV have on NK cells without therapy? (1)

A

Reduced NK function

OD2 S1 (47 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions