Lysosomes

Question 1

the pH of the inside of the lysosome, how is it created

Answer 1

5- allows for dissociation of the cargo (enzymes), H+ proton pumps

Question 2

Describe the endocytic pathway

Answer 2

clathrin coated pit and receptor-mediated endocytosis, fusion with early endosomes in a cluster, late endosome formation, lysosome formation

Question 3

2 characteristics used to identify lysosome

Answer 3

1. pH 4.5 in lumen

2. Presence of hydrolases and lysosomal associated membrane proteins (LAMPs)

Question 4

cis-Golgi role in lysosome process

Answer 4

phosphorylation of mannose residues on the lysosomal enzymes

Question 5

trans-Golgi role in lysosome process

Answer 5

sorting of the phospho-enzymes destined for lysosomes and vesicular transport