lysosome

Question 1

deliver membrane proteins to lysosome with

Answer 1

multivesticular endosome

Question 2

deliver cytosolic proteins to lysosome with

Answer 2

autophagy

Question 3

multivesticular endosome mech

Answer 3

Ubiquitin- tag for cargos going into MVB (monounbiquitin), ESCRT complex is recruited by
ubiquitn-tagged Hrs protein, ESCRT proteins drive vesicle budding
into the lumen, loading of
monoubiquitinated membrane cargos
into vesicle buds, pinching off, Vps4, an ATPase, disassemble
ESCRT complex, ESCRT and Vps4 function as SNARE
and NSF

Question 4

budding viruses

Answer 4

sim to MVB but uses Gag molecules to form a shell, Tsg101 (ESCRT protein) binds to Gag

Question 5

autophagy

Answer 5

recycling of macromolecules for use as nutrients under stress,
such as starvation

Question 6

autophagy mech

Answer 6

formation of flattened double-membrane cup shaped structure to envelop an entire organelle, outer membrane of autophagic vesicles fuse with lysosome, Enzymes in lysosome
degrade the autophagic
vesicles