Lymph node immuno

Question 1

Immuno of CLL/SLL

Answer 1

+ve CD5,19,20,79b,23, surface light chain
-ve CyclinD1,
CD38, ZAP-70 expression poor prognostic factors

Question 2

Immuno of mantle cell lymphoma

Answer 2

+ve CD5,19,20,79a/b, BCL2, cyclin D1, Surface IgM heavy chain (+/-IgD)
-ve CD10, BCL6, MUM1, CD23
Ki67 low/mod

Question 3

Immuno of lymphoplasmacytic lymphoma

Answer 3

+ve CD19, 20, 79a, BCL2, PAX5, surface IgM,IgD

-ve CD3,5,10,23, BCL6, CD138

Question 4

Immuno of splenic marginal zone lymphoma

Answer 4

+ve CD19, 20, 79b, surface IgM and IgD

-ve CD 5, 10, 23, Cyclin D1

Question 5

Immuno of hairy cell leukaemia

Answer 5

+ve TRAP, CD19, 20, 22, 25, 11c, 103, 123, surface IgG
Cyclin D1 50% +ve
-ve CD79b, CD5, 10, 23

Question 6

Immuno of plasma cell myeloma

Answer 6

+ve CD38, 138, 56, MUM-1, light chain restriction
CD19 weak +ve, CD79a variable
-ve CD 45, CD20 (expression related to t(11;14)

Question 7

Immuno of Burkitt lymphoma

Answer 7

+ve CD 19, 20, 79a, 10, BCL6,
-ve CD5, 23, BCL2, MUM-1, CD45, TdT,
Ki67 100%

Question 8

Immuno of follicular lymphoma

Answer 8

+ve CD19, 20, 79a, 10, BCL2, BCL6, surface Ig DMG
CD21 to assess follicular areas, Ki67 variable
-ve 23 (usu), Cyclin D1, MUM-1 (may be +ve in higher grade ones)

Question 9

Immuno of DLBCL

Answer 9

B cell markers +ve (inc PAX-5), surface IgM, IgG
CD10, BCL2, MUM-1 depending on subtype
-ve CD23, CD5, cyclin D1, variable BCL2
Plasmablastic variant +ve CD56, light chain, p63
Ki67 >30%

Question 10

Immuno of Extranodal NK/T cell lymphoma, nasal type

Answer 10

+ve CD 2, 5, 7, 45, 56, granzyme, perforin, TIA-1, EBER

-ve CD3, 4, 7, 8, TCRb, TdT

Question 11

Immuno of EATL

Answer 11

+ve CD2,3,7, granzyme, perforin, TIA-1
CD30 variable
-ve CD4,5,8, 56, EBER