Lymph node development

Question 1

Which chemokine-chemokine receptor pair is mediating the initial clustering of LTi cells during the initiation phase of lymph node development?

Answer 1

CXCL13 with CXCR5 and CCL21 with Ccr7

Question 2

How are the lymph node stromal cells called that attack Tcells and which chemokines are involved?

Answer 2

FRC = fibroblastic reticular cells > Tcell area

Chemokines =

Question 3

Which cytokine and its receptor are essential for LN development?

Answer 3

Lymphotoxina1b2 and LTbR are essential for lymphoid organ formation

Question 4

Which cells are necessary to form lymph nodes?

Answer 4

Hematopoetic cells (immune cells) and stromal cells (non-immune cells)

Question 5

In embryonal development, when does the cluster of stromal cells and immune cells start forming?

Answer 5

8 weeks

Question 6

Describe adaptive immunity with lymph nodes in the answer

Answer 6

In the lymph nodes the adaptive immune response starts, the antigen is presented to the lymphocyte here. DC in the periphery picks up an antigen and brings it to the lymph nodes, those Tcells can recognize it and will respond > generate adaptive immune response and go to the site of damage and help immune response

Question 7

Why do we need lymph nodes?

Answer 7

• Protection: initiation adaptive immunity
• Memory response
• Control of self-reactive lymphocytes > within the lymph nodes

Question 8

Which cytokines are important for the formation of the immune system?

Answer 8

Lymphotoxin alpha1beta2 and TNF

Question 9

Name 2 functions of TNF

Answer 9

Formation immune system

Involved in inflammation

Question 10

How are the lymph node stromal cells called that attract Bcells and which chemokines are involved?

Answer 10

FDC = follicular dendritic cells > B-cell follicles

Chemokines involved are =

Question 11

Which cells produce retinoic acid?

Answer 11

RALDH is expressed in nerve fibers

Question 12

Upon experimental infection of the salivary gland, tertiary lymphoid structures appear as organized clusters of immune cells. This formation critically depends on the production of IL22.
2a) How could you study the importance of IL22 for the formation of the tertiary lymphoid structure in this disease model. Give 2 options and explain your answer.

Answer 12

.

Question 13

2b) Are lymphoid tissue inducer cells involved in the formation of these tertiary lymphoid structures? Explain your answer.

Answer 13

.