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CC1 > Liver Function Test > Flashcards

Liver Function Test Flashcards

1
Q

Perform majority function of liver;
Degenerative property of liver

A

Hepatocyte

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2
Q

Act as phagocyte

A

Kupffer cells

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3
Q

Line the sinusoid of the liver

A

Kupffer cells

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4
Q

Large organ divided unequally into 2 lobules by

A

Falciform ligament

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5
Q

Functional unit of the liver

A

Lobules

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6
Q

Chief metabolic organization

A

Liver

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7
Q

Responsible for all metabolic and excretory function of liver

A

Lobules

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8
Q

Amount of blood supply in the body

A

1500 ml/minute

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9
Q

Function of liver

A
  1. Synthetic function
  2. Conjugate function
  3. Detoxification and drug metabolism
  4. Excretory and secretory function
  5. Storage function
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10
Q

Ability to synthesize substance (protein)

A

Synthetic function

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11
Q

Metabolism of bilirubin

A

Conjugation function

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12
Q

Bile (elimination of cholesterol and bile salts)

A

Excretory and secretory function

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13
Q

Fat soluble

A

Vitamin A, D, E, K

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14
Q

Albumin + Globulin

A

Total protein

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15
Q

Sample used in total protein

A

Serum

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16
Q

Interference in total protein

A

Hemolysis and ictericia

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17
Q

Reference value for Total protein

A

6.5 - 8.3

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18
Q

Assessment of nutritional status and presence of diagnosis of disease involving kidney, liver and bone marrow

A

Total protein

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19
Q

Methods in total protein

A
  1. Kjeldahl
  2. Biuret
  3. Folin - Ciocalteu (lowry)
  4. UV absorption
  5. Refractometry
  6. Turbidimetry and Nephelometry
  7. Commasie brilliant blue dye
  8. Ninhydrin
  9. Serum protein electrophoresis
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20
Q

Classical method; reference but not routinely used

A

Kjeldahl

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21
Q

Colorimetric non enzyme method

A

Biuret

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22
Q

3 steps in kjeldahl method

A

Digestion
Distillation
Titration

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23
Q

Oxidation of phenolic compounds such as ___

A

Tyrosine, tryptophan & histidine

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24
Q

Principle: oxidation of phenolic compounds

A

Folin - ciocalteu (lowry) method

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25
Q

Based on refractive index of serum

A

Refractometry

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26
Q

Absorbance used in UV absorption method

A

210 nm

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27
Q

Absorption @ 280nm

A

Tryptophan, tyrosine & phenylalanine

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28
Q

Measures the amount of nitrogen

29
Q

1g of nitrogen =

A

6.54 g of protein

30
Q

Uniform fine precipitate which scatter lights or block of light

A

Nephelometry and turbidity

31
Q

Detection of protein as little as 1 ug; disbonding technique

A

Coomasie brilliant blue dye

32
Q

Color formed in biuret method

A

Deep purple / violet

33
Q

Most widely used method for total protein

A

Biuret method

34
Q

Biuret method absorbance

35
Q

Based on peptide bond present in protein with cupric ions

36
Q

Interference in biuret method

37
Q

Reagents used in biuret method

A

Alkaline copper sulfite
Sodium potassium tartrate (rochelle salts)
NaOH
KI (stabilizer)

38
Q

Color formed in Folin- ciocalteu

39
Q

High analytical sensitivity

A

Folin-ciocalteu

40
Q

Main reagent in folin

A

Phosphotungstic - molybdic acid/ phenol reagent

41
Q

Folin color enhancer

A

Biuret reagent

42
Q

Violet color reacting with primary amines widely used for detecting peptides and amino acids after paper chromatography

43
Q

Either positive or negative

A

Amphoteric

44
Q

Migration of charged particles in an electric field

45
Q

SPE buffer

A

Barbital (veronal)

46
Q

pH of buffer

A

6.8 (basic)

47
Q

Reference value of albumin bond

A

3.5-5.0 g/dl (56-65%)

48
Q

Reference value of a1 globulin

A

0.1-0.3 g/dl (2.5-5%)

49
Q

Reference value of a2 globulin

A

0.6-1.0 g/dl (7-13%)

50
Q

Reference value of beta globulin

A

0.7 - 1.1 g/dl (8-14%)

51
Q

Gamma globulin reference value

A

0.8-1.6 g/dl (12-22%)

52
Q

The concentration is directly proportional to the severity of hepatic disease

53
Q

Methods in albumin

A

Salt precipitation
Dye binding

54
Q

Albumin is precipitated?

A

No. Globulin is precipitated

55
Q

Albumin is quantitated using biuret reaction?

56
Q

Binds to positive charge

A

Anionic dye

57
Q

Dye commonly used

A

Bromocresol green

58
Q

Most specific dye

A

Bromocresol purple

59
Q

Not specific to albumin

A

Methyl orange

60
Q

More specific to albumin

A

HABA - 2,4 - Hydroxyazobenzene - benzoic acid

61
Q

Interference in dye binding

A

Conjugated bilirubin
Aspirin
Sulfonamides

62
Q

Reference value of A/G ratio

A

1.3-3.1 g/dl

63
Q

Formula of AG ratio

A

AG ratio = albumin / globulin

Globulin = total protein - albumin

64
Q

Vitamin k response test

A

Prothrombin time

65
Q

Amount administered in PT

A

10 mg for 1-3 days

66
Q

It differentiate intrahepatic from extra hepatic disorder

67
Q

Prolonged PT

A

Intra hepatic

68
Q

Not prolong PT

A

Extra hepatic

CC1 (3 decks)

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