Lipoprotein Flashcards
Free Fatty acid or NEFA is …
Complex with albumin
They are not included in the classification of lipoprotein
Classification
- Chylomicrons ——- apoprotein B 48
- VLDL ( very low density lipoproteins main apo protein is B 100
- Intermediate density lipoprotein (IDL)
- Low density lipo protein (LDL) beta lipoprotein major apoprotein B100
- High density lipoprotein (HDL) or alpha lipoprotein major apoprotein is apo A
The lipoprotein are characterized on the basis of …
Their density . Fat is less dense than water so fat float on water
Lipoprotein electrophoresis
The serum is applied on cellulose acetate , electric current is applied for 2 hours , the strip is dried and stained with lipid dyes such as oil red o . As a general rule those with higher protein content will move faster towards the anode and those with less proteins have minimum mobility
Apolipoprotein
The protein pat of lipoprotein is called apolipoprotein or apoprotein
All apoproteins are mainly synthesis in liver but small quantities are produce from almost all organs
Intestinal cells produce small quantities of apo A
Apo A-I
Activate lecithin- cholesterol acyl transferase (LCAT)
It is the ligand for HDL receptor
It is anti atherogenic
It is specific for HDL
Apo B 100
Only apoprotein component of LDL it binds to LDL receptor on tissues
Apo B 100 is one of the biggest proteins , having 4536 amino acid with the molecular weight of 550 KDa
It is synthesis in the liver
Apo B 48
Major apoprotein of chylomicrons
It is synthesis only in intestinal cells
Apo B 100 and apo B 48 are products of the same gene but in the intestine the mRNA undergoes editing so as to produce the B48 protein
Apo C II
Activates lipoprotein lipase
Apo E
Arginine rich protein
It is present in chylomicrons LDD and VLDL
Astrocytes also make apo E it is involved in cellular transport of lipids in cCNS apo E has 4 isoforms
