Lipids

Question 1

A student investigated the effect of lipase concentration on the hydrolysis of lipids. The student did not add a buffer to the lipase solution. Explain why. [1]

Answer 1

student was measuring a change in pH;
OR buffer would maintain a constant pH;

Question 2

Lipids are in suspension. Give two variables the student would have controlled in this investigation. [2]

Answer 2

Volume of suspension of lipids;
Concentration of suspension of lipids;
Volume of lipase solution;
Temperature;

Question 3

Give the suitable control for this investigation. [1]

Answer 3

boiled lipase solution;

Question 4

The student repeated the experiment with a higher concentration of lipase solution. Describe and explain the results you would expect him to get in terms of pH. [3]

Answer 4

faster fall in pH but levels off in same place;
enzyme substrate complexes formed quickly;
same amount of fatty acids produced;