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CC2024 > LIPID AND LIPOPROTEIN ANALYSES > Flashcards

LIPID AND LIPOPROTEIN ANALYSES Flashcards

1
Q

Principle: Dehydration and oxidation of cholesterol to form a colored compound (colorimetric)
A. CHOLE MEASUREMENT
B. TRIGLY MEASUREMENT
C. LIPOPROTEIN MEASUREMENT

A

A. CHOLE MEASUREMENT

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2
Q

**CHOLESTEROL **

SAMPLE SHOULD BE
A. PLASMA
B. SERUM
C. BOTH A AND B
D. EDTA

A

BOTH A AND B

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3
Q

CHOLESTEROL PATIENT SHOULD HAVEA A
_ week prior testing with in a usually diet

A. 3 WEEKS
B 1 WEEK
C. 2 WEEKS

A

C. 2 WEEKS

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4
Q

**SAMPLE SUBMITTED FOR CHOLESTEROL MEASUREMENT SHOULD BE **
A. NON FASTING BLOOD SAMPLE
B. FAST BLOOD SAMPLE

A

A. NON FASTING BLOOD SAMPLE

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5
Q

Non-Enzymatic Method FOR CHOLESTEROL

A. ABELL KENDALL
B. CHOLESTEROL OXIDASE

A

A. ABELL KENDALL

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6
Q

Principle: Dehydration and oxidation of cholesterol to form a colored compound

A. NON-EZYMATIC ABELL KENDALL
B ENZYMATIC ABELL KENDAL

A

A. NON-EZYMATIC ABELL KENDALL

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7
Q

One step method
A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

A

A. COLORIMETRY

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8
Q

**Two step method IN ABEL KENDALL **

A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

A

B. extraction followed by colorimetry

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9
Q

3 step method IN ABEL KENDALL
A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

A

C. extraction, saponification, and colorimetry

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10
Q

4 step method IN ABEL KENDALL
A. COLORIMETRY
B. extraction followed by colorimetry
C. extraction, saponification, and colorimetry
D.precipitation, saponification, extraction, and
colorimetry

A

D.precipitation, saponification, extraction, and
colorimetry

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11
Q

Cholesteryl esters are hydrolized with

A. HEXANE
B. ALCOHOLIC KOH

A

alcoholic KOH

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12
Q

Unesterified cholesterol is extracted with
A. HEXANE
B. ALCOHOLIC KOH

A

HEXANE

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13
Q

** THE NON ENZYMAIC METHOD OF ABELL KENDALL IS Measured using:**

A. Liebermann-Burchard reaction
B. Brodie method

A

A. Liebermann-Burchard reaction

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14
Q

NON ENZYMATIC

❖ Cholesterol + sulfuric acid + acetic anhydride→

A. BLACK
B. GREEN (CholestadienylMonosulfonicAcid)
C. RED SOLUTION . (CholestadienylDisulfonic Acid)

A

B. GREEN

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15
Q

Salkowski reaction:

A. BLACK
B. GREEN
C. RED SOLUTION

A

C. RED SOLUTION
. (CholestadienylDisulfonic Acid)

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16
Q

Cholesterol oxidase
A. Enzymatic Method
B. NON ENZYMATIC METHOD

A

A. Enzymatic Method

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17
Q

Enzymatic Method

H2O2 + Dye –Peroxidase -> WHAT COLOR OF THE PRODUCT (Quinoneimine dye)

A

VIOLET

18
Q

Cholesterol + O2 –Cholesterol oxidase - >

A. 4-cholestenone + h2o2
B. cholesterol + fatty acid

A

A. 4-cholestenone + h2o2

19
Q

Total Cholesterol (cholesteryl ester and cholesterol) + H2O –Cholesteryl esterase ->

A. 4-cholestenone + h2o2
B. cholesterol + fatty acid

A

B. cholesterol + fatty acid

20
Q

**TRIGLYCERIDE FASTING ONLY
→ There is a strict fasting for - hours **

A.12 HRS
B 10 HS
C. 8 HRS

A

A.12 HRS

21
Q

Triglycerides with FBS (fasting blood glucose) fasting for HOW MAY hrs is accepted

A.10HRS
B. 12 HRS

A

A.10HRS

22
Q

NON ENZYMATIC FOR TRIGLY MEASUREMNT

FIRST. Triglyceride –Alcoholic KOH →

A. + Glycerol + Fatty acid
B. Formaldehyde

A

A. Glycerol + Fatty acid

23
Q

NON ENZYMATIC FOR TRIGLY MEASUREMENT

2ND. Glycerol + Periodic acid →

A. FORMALDEHYDE
B. + Glycerol + Fatty acid

A

A. FORMALDEHYDE

24
Q

**Van Handel and Zilversmith **
Formaldehyde + Chromotropic acid → Blue Solution

A, Colorimetric Method)
B. Fluorometric Method)

A

A, Colorimetric Method)

25
Q

**Hantzch Condensation **

Formaldehyde + Diacetyl acetone + NH3 → Yellow Solution

A. COLORIMETRIC
B. FLUOROMETRIC METHOD

A

B. FLUOROMETRIC METHOD

26
Q

Enzymatic Methods FOR TRIGLY
A. : Glycerol Kinase
B. : Abell Kendall

A

A. : Glycerol Kinase

27
Q

ADP + PEP –Pyruvate kinase ATP+ Pyruvate Pyruvate + NADH + H+ –LD–>* Lactate* + NAD+ (340 nm)

A. PYRUVATE KINASE CHOLE
B PYRUVATE KINASE TRIGLY

A

B PYRUVATE KINASE TRIGLY

28
Q

Glycerophosphate + O2 –GO → **+ Dihydroxyacetone **+ H2O2
o H2O2 + Dye –Peroxidase → Color

A. PYRUVATE KINASE CHOLE
B PYRUVATE KINASE TRIGLY
C. GLYCEROL- PHOSPHATE OXIDASE

A

Glycerol-Phosphate Oxidase

29
Q
A
30
Q

CDC REFERENCE METHOD: Modified Van handheld and SilversmitH

A. TRIGLY
B. CHOLE

A

A. TRIGLY

31
Q

LIPOPROTEIN
MEASUREMENT Preferred sample

A. SERUM SST
B. EDTA

A

A. SERUM SST

32
Q

**For Research Purposes FOR LIPOPROTEIN **
A. SST SERUM
B. EDTA PLASMA
C EDTA SERUM

A

B. EDTA PLASMA

33
Q

LIPOPROTEIN MEASUREMENT

TRUE OR FALSE
For non-fasting sample: total cholesterol and HDL can be measured but not LDL and VLDL

A

TRUE

34
Q

LIPOPROTEIN

Ultracentrifugation SOLUTIO REAGENT SG

A. Potassium Bromide with a specific gravity fixed at
1.063

B.Potassium Bromide with a specific gravity fixed at
1.064

A

A. Potassium Bromide with a specific gravity fixed at
1.063

35
Q

Order from the lightest to heaviest:

A

Chylomicrons →VLDL →LDL →HDL

36
Q

COLLECTED SUPERNATE IN THE CENTRI
A. HDL
B LDL
C. VLDL

A

HDL

37
Q

PRECIPITATE WHICH LEFT IN THE TUBE

A. HDL
B LDL
C. VLDL

A

LDL
C. VLDL

38
Q

Lipid Profile Test contains

a. total cholesterol
b. triglycerides
c. HDL, LDL, and
VLDL
d. all

A

all

39
Q

are not directly measured (not usually measured)
a. ldl and vldl
b. total chole, trig, hdl

A

a. ldl and vldl

40
Q

– for determination of LDL and VLDL conc.

a. apo B
B. APO A-1

A

a. apo B

41
Q

for determination of HDL conc.

a. apo B
B. APO A-1

A

B. APO A-1

CC2024 (18 decks)

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