Link reaction and Kreb's cycle

Question 1

where does the link reaction and Kreb’s cycle take place?

Answer 1

mitochondrial matrix
- pyruvate and NADH actively transported from the cytoplasm

Question 2

link reaction

Answer 2

• pyruvate from glycolysis oxidised into acetate
• NAD picks up H+ and becomes NADH and a CO2 is released
• acetate combines with CoA to produce acetylcoA
• occurs twice for every molecule of glucose

Question 3

products of the link reaction

Answer 3

2 x acetylcoA
2 x CO2 released
2 x reduced NAD

Question 4

Kreb’s cycle

Answer 4

• acetylcoA reacts with oxaloacetate (4C) molecule, releases coA (reused in LR), producing citrate (6C) that enters KC
• CO2 lost to produce a 5C molecule
• series of redox reactions,
• krebs cycle generates reduced coenzyme and ATP by substrate level phosphorylation

Question 5

products per glucose molecule

Answer 5

6 x NADH
2 x reduced FAD
2 x ATP
2 x CO2