Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Biology > Light and Electron Microscope > Flashcards

Light and Electron Microscope Flashcards

You may prefer our related Brainscape-certified flashcards:
AP® Biology flashcards Biology 101 flashcards
1
Q

What is magnification?

A

How big

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

How do you work out total magnification of a microscope?

A

Eyepiece x objective lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What would the total magnification of a microscope with x10 eyepiece and x100 objective lens be?

A

10 x 100 = x1000 magnification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is resolution?

A

The ability to distinguish between two points e.g. with a maximum resolution of 200nm, objects have to be 200nm apart to distinguish between them

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What does a light microscope use to magnify small objects?

A

Visible light and lenses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the wavelength of visible light?

A

400nm –> 700nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Do light microscopes give 2D or 3D images?

A

2D

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the maximum magnification of a light microscope?

A

x1500 (in school we have x1000)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the maximum resolution of a light microscope?

A

200nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Why is the maximum resolution of a light microscope 200nm?

A

It’s about half the wavelength of visible light

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What lights give the best and worst resolutions?

A

Blue light - best
Red light - worst

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

How do electron microscopes work?

A

Electrons travel in waves and are focused using electromagnets

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What dimensions can electron microscopes create images in?

A

2D and 3D

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the maximum resolution of an electron microscope?

A

x50,000
(normal electron microscope x250,000)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the maximum resolution of an electron microscope?

A

0.5nm
(400x better)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

How does a scanning electron microscope (SEM) work?

A

Causes electrons to bounce off of specimen

14
Q

Does an SEM produce 2D or 3D images?

A

3D

15
Q

How does a transmission electron microscope (TEM) work?

A

Sends electrons through specimen

16
Q

Does an TEM produce 2D or 3D images?

A

2D

17
Q

Give 4 advantages of electron microscopes

A

1- higher resolving power
2- higher resolution
3- heavy metal stains can highlight key structures
4- false colour can be added with computers

18
Q

Give 2 disadvantages of electron microscopes

A

1- not portable
2- vacuum required so specimen can’t be alive

19
Q

Give 3 disadvantages of TEMs

A

1- prep of specimen is difficult and time consuming (need to ensure it’s thin)
2- prep machine is expensive
3- prep can disturb organelle and cause artifacts (tissue folds on itself)

Biology (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions