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Biology > Light and Electron Microscope > Flashcards

Light and Electron Microscope Flashcards

1
Q

What is magnification?

A

How big

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2
Q

How do you work out total magnification of a microscope?

A

Eyepiece x objective lens

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3
Q

What would the total magnification of a microscope with x10 eyepiece and x100 objective lens be?

A

10 x 100 = x1000 magnification

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4
Q

What is resolution?

A

The ability to distinguish between two points e.g. with a maximum resolution of 200nm, objects have to be 200nm apart to distinguish between them

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5
Q

What does a light microscope use to magnify small objects?

A

Visible light and lenses

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5
Q

What is the wavelength of visible light?

A

400nm –> 700nm

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6
Q

Do light microscopes give 2D or 3D images?

A

2D

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7
Q

What is the maximum magnification of a light microscope?

A

x1500 (in school we have x1000)

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8
Q

What is the maximum resolution of a light microscope?

A

200nm

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9
Q

Why is the maximum resolution of a light microscope 200nm?

A

It’s about half the wavelength of visible light

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10
Q

What lights give the best and worst resolutions?

A

Blue light - best
Red light - worst

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11
Q

How do electron microscopes work?

A

Electrons travel in waves and are focused using electromagnets

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12
Q

What dimensions can electron microscopes create images in?

A

2D and 3D

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13
Q

What is the maximum resolution of an electron microscope?

A

x50,000
(normal electron microscope x250,000)

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13
Q

What is the maximum resolution of an electron microscope?

A

0.5nm
(400x better)

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14
Q

How does a scanning electron microscope (SEM) work?

A

Causes electrons to bounce off of specimen

14
Q

Does an SEM produce 2D or 3D images?

A

3D

15
Q

How does a transmission electron microscope (TEM) work?

A

Sends electrons through specimen

16
Q

Does an TEM produce 2D or 3D images?

A

2D

17
Q

Give 4 advantages of electron microscopes

A

1- higher resolving power
2- higher resolution
3- heavy metal stains can highlight key structures
4- false colour can be added with computers

18
Q

Give 2 disadvantages of electron microscopes

A

1- not portable
2- vacuum required so specimen can’t be alive

19
Q

Give 3 disadvantages of TEMs

A

1- prep of specimen is difficult and time consuming (need to ensure it’s thin)
2- prep machine is expensive
3- prep can disturb organelle and cause artifacts (tissue folds on itself)

Biology (5 decks)

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