Life cycle of SARS-COV 2

Question 1

SARS-COV2 life cycle requirement

Answer 1

• requires ATP

Question 2

ENTRY

Answer 2

• Viral protein needs to get in so it can access ribosomes and mitochondria

Occurs via 2 stages:
• Receptor mediated endocytosis (RME)
• Fusion at PM (virus triggers membrane fusion process)

• Virus needs to find cellular protein to get into the cell
• For SARS-COV2 it is ACE-2

Question 3

What is RME?

Answer 3

• Receptor mediated endocytosis
Bulk amounts of specific molecules can be imported into a cell after binding to cell surface receptors
• Molecules which are bound – taken into cell via INWARD folding of cell surface membrane
• Cell surface membrane is PINCHED OFF into a vesicle within the cell

Question 4

REPLICATION

Answer 4

• Building blocks of virus are made and RNA is copied

Occurs via:
• Viral RNA translation to make proteins
• Genome replication to make more viruses
• Assembly

Question 5

EGRESS

Answer 5

Occurs via:
• Envelopment
• Exocytosis mediated egress