Library bases synthesis

Question 1

Furkas mix and split technique

Answer 1

1. link each AA to an encoded resin 2. mix beads together and split into three equal portions 3. react each portion wit a different amino acid (encode) 4. isolate all beads, mix together and split into three equal portions 5. react ach portion with a different AA (ENCODE) 6. seperate each encoded bead, decode, cleave and filter

Question 2

Houghton’s teabag procedure

Answer 2

• portions of resin are placed in polypropylene mesh containers - seperate reactions are carried out by placing each bag in polyethylene bottles containing reagents -tea bags make it easy to wash resins between steps and can be combined for common proceadures i.e. deprotection or washes

Question 3

RF encoding

Answer 3

• beads are microchipped and can be tracked

Question 4

Peptides as compounds

Answer 4

• good diversification (amino acid substrates ) - good 3D spatial orientation into binding domains and pharmacophoric complementarity to proteins - poor adme properties

Question 5

Peptoids

Answer 5

• peptide like molecules that use SPSS technology but have better ADME compliance

Question 6

Heterocycles

Answer 6

better adme properties and can be adapted to SPSS

Question 7

Difference between peptoids and peptides

Answer 7

Question 8

Reagents used to promote cleavage

Answer 8