Leukaemia Flashcards
What are the main types of leukaemia?
Acute myeloid leukaemia
Acute lymphoblastic leukaemia
Chronic myeloid leukaemia
Chronic lymphoid leukaemia
What is the pathophysiology of ALL?
Arises from lymphoid progenitor cells that undergo malignant transformation
Most are B cell in origin
May arise from T cells
As clonal expansion occurs, these precursors replace other haematopoietic cells in the bone marrow
Classified as B cell lineage
T cell lineage
What cytogenic features are seen in ALL?
t(12;21) most common translocation seen in children - TEL-AML fusion gene
t(9;22) Philadelphia chromosome - BCR-ABL
t(4;11) in infants <12 months, rare in adults
Hyperdiploid karyotype
Hypodiploid karyotype
What are the clinical features of ALL?
Marrow suppression symptoms and lymphadenopathy
Marrow failure:
Anaemia - fatigue, SOB, angina
Neutropenia - recurrent infections
Thrombocytopenia - petechiae, nose bleeds, bruising
Tissue infiltration Lymphadenopathy Hepatosplenomegaly Bone pain Mediastinal mass - may result in SVCO Testicular enlargement
Leucostasis
May occur due to large numbers of WCs entering blood stream
Organ dysfunction due to impairment through small vessels
Altered mental state, headache, SOB, visual changes
What is the presentation of T cell ALL?
Rarer than B cell form
Typically present in adolescent males
With lymphadenopathy or a mediastinal mass
What are the investigations for ALL?
Bloods
FBC, U&Es, LFTs, clotting
DDIMER, bone profile,
uric acid, LDH, BBV
Most will present with pancytopenia
Uric acid and LDH non specific markers of tumour border
DDIMER and coag for DIC
Imaging
CXR - mediastinal mass
CT chest, abdo pelvis for lympadenopathy and organ involvement
CT/MRI head exclude differentials, neurology
Bone marrow aspiration and biopsy, staining and review of cell morphology
Immunophenotyping
Blood smear
Pleural tap if pleural effusion
LP if CNS involvement
What are high-risk factors for adult patients, indicating a poorer prognosis?
Age - worse with advancing age Performance status > 1 WCC - >30 for B, >100 for T Cytogenetics - 9;22, 4;11 Immunophenotype - proB, early and mature T CNS involvement
What is the management of ALL?
Referral to haemato-oncology specialists
- Pre-phase therapy - steroids, with allopurinol and IV hydration, reduces risk of TLS
Can give rasburicase to prevent TLS as helps clears uric acid from body
Leucopheresis can help reduce WCC for TLS.
Anaemia and thrombocytopenia may need treatment, G-CSF for neutropenia.
- Induction chemotherapy
For complete remission or molecular complete:
Complete - not in bone marrow, peripheral blood or CSF
MolecularCR - minimal disease not detectable by sensitive molecular probe - Maintenance therapy
Daily 6-mercaptopurine
Weekly methotrexate
Reduce risk of recurrence - Stem cell transplant
Allogenic
Myeloablative transplant = high dose chemo, then stem cell transplant - Palliative care
What complications are ALL patients at risk of?
TLS
Neutropenic sepsis
SVCO - dyspnoea, facial swelling, cough secondary to mediastinal mass
Chemo side effects - early mucositis, nausea, vomiting, hair loss or late - cardiomyopathy, secondary malignancies
What do you look for in a cytochemical stain in acute leukaemias?
TdT+ in lymphoblasts - ALL
Myeloperoxidase in myeloblasts - AML
How is philadelphia + ALL managed?
Stem cell transplant
Imatinib
TKI’s - tyrosine kinase inhibitors
What is APL? What genetics is it associated with?
Acute Promyelocytic Leukaemia
translocation of chromosome 15 and 17 t(15;17)
How does APL normally present? Describe the pathophysiology of this
Younger than AML and it’s other subtypes (age 25 ish)
build up of promyelocytes –> lots of Auer rods –> high coagulation risk –> DIC
Medical Emergency!
How is APL treated?
It responds to retinoic acid (ATRA)
What is myelodysplastic syndrome?
What would the bone marrow look like?
How would it present?
Condition which is a precursor of AML
Blasts build up in marrow but <20%
Same signs - cytopaenia, bruising etc.
Describe the age presentation of ALL vs AML
ALL is childhood (2-5)
AML is adults (65)
What are the blood results in ALL?
normocytic, normochromic anaemia
thrombocytopenia
leukocytosis but with neutropenia
reduced reticulocytes
Renal failure: raised K and phosphate
raised LDH
What is the bone marrow like in ALL?
hypercellular
blast cell infiltration
On cytochemical staining, what is seen in ALL? Compare this to AML?
blasts are TDT+ve
In AML it is myeloperoxidase +ve
What is ALL associated with?
Most common in children
Associated with Down’s
What is CLL associated with?
Most common leukaemia in adults overall
Associated with warm haemolytic anaemia
Richter’s transformation in lymphoma
Smudge/smear cells
What is CML associated with?
Has three phases
A 5 year - ‘asymptomatic chronic phase’
Associated with the Philadelphia chromosome
What is AML associated with?
Most common adult acute leukaemia
Can be the result of a transformation from a myeloproliferative disorder
Associated with Auer rods
What are the differentials for petechiae?
Leukaemia Meningococcal sepsis Vasculitis Henoch Schonlein Purpura Idiopathic Thrombocytopenia Purpura Non accidental injury
What is the pathogenesis of a haematological malignancy?
Cause - environment, toxin, virus infection, drug, genetic predisposition: translocation, deletion, duplication, point mutation
Pathogenesis - altered gene expression, change in oncogene or tumour suppressor gene
Leads to decrease in apoptosis and differentiation, and proliferation increase
What are lymphoid haematological malignancies?
Acute - lymphoblastic leukaemia
Chronic Lymphocytic leukaemia Non-Hodgkin lymphoma Hodgkin lymphoma Multiple myeloma
What are myeloid malignancies?
Acute myeloid leukaemia
Chronic myeloid leukaemia
Myelodysplasia
Myeloproliferative disorders e.g. polycythaemia vera, essential thrombocytopenia, primary myelofibrosis
What would be shown on a blood film in T-ALL?
Bone marrow showing large number of lymphoblasts with a high nuclear/cytoplasmic ratio
What would be shown on a blood film in B-ALL?
Large blasts with characteristic vacuoles and blue cytoplasm
What are prognostic indicators in AML suggesting a better outcome?
Cytogenic changes - 8;21, 15;17, inversion 16
Remission after one course of chemotherapy