Let's Go Flashcards
3 things that make up the pre-initiation complex?
DnaA: ATPases melt Ori open
DNA Helicsase
SSBs
Which topoisomerase requires ATP?
II!
Ciproflaxin
mixed mode, non-competitive inhibitor of DNA gyrase
Which subunit of DNA Pol III forms ring?
Beta
Does DNA ligase use ATP?
yes
What removes primers in eukaryotic replication?
RNase H and FEN1
Pol Alpha
primase
Pol Beta
repair
Pol gamma
replicates mitochondrial DNA
Pol delta
lagging synthesis
Pol Epsilon
leading synthesis
Nucleoside Analogs
AZT (thymidine)
2,3 dideoxyinosine (deoxyadenosine)
modified 3’OH on ribose
What are 4 mistakes of DNA?
mismatch repair
thymidine dimers
base excision repair
double strand breaks
Lynch Syndrome
mutation in Mut
Homologous Recombination Repair
sister chromatids used as template to repair missing end for a double strand break - BRCA - component
Non-Homologous End Joining
protein complex brings 2 duplex strands end-to-end and a DNA ligase joins them; no template used
UV Specific Endonuclease
XP Protein
Eukaryotic RNA Pols
RNA Pol 1 = transcribes rRNA
RNA Pol 2 = transcribes mRNA
RNA Pol 3 = transcribes tRNA & sn RNA
TFIID
recognizes and binds TATA box - deez TATAs
TFIIF
interacts and recruits RNA Pol II to promoter
TFIIH
helicase activity to unwind DNA at transcription start site
HAT
enzyme for histone acetylation
HDAC
histone deacetylase
snoRNAs
base and sugar modifications, help interaction with protein components of ribosome; processing in nucleolus
