Antibiotics

Question 1

Streptomycin

Answer 1

prokaryotes, initiation

binds to 30S subunit, distorting it

Question 2

Tetracyclines

Answer 2

prokaryotes, elongation

Binds to 30S subunit, blocks entry of AA-tRNA to A side

Question 3

Chloramphenicol

Answer 3

prokaryotes, elongation,

Inhibits peptidyltransferase

Question 4

Clindamycin

Erythromycin

Answer 4

prokaryotes, elongation,

Binds to 50S subunit, blocks translocation

Question 5

Diptheria Toxin

Answer 5

eukaryotes, elongation,

inactivates elongation factor, blocks translocation

Question 6

Cycloheximide

Answer 6

eukaryotes, elongation

may bind to E site and block translocation

Question 7

Puromycin

Answer 7

both, elongation

AA-tRNA analogue, causes peptide chain termination

Question 8

Bevacizumab (Avastin™)

Answer 8

monoclonal antibody that binds VEGF and blocks it from binding to VEGF receptors on the surface of vascular endothelial cells.

Question 9

Name the 3 drugs that inhibit the kinase activity of VEGF-R

Answer 9

Sunitinib (Sutent™)
Sorafenib (Nexavar™)
Cederanib (Recentin™ - AZD- 2171)

Question 10

Rifampicin

Answer 10

used in Tb, inhibits initiation of transcription in prokaryotes
binds to Beta subunit of holoenzyme, inhibits formation of first phosphodiester bond

Question 11

Actinomycin D

Answer 11

chemotherapy

inhibits elongation of RNA transcription.. binds to DNA

Question 12

a-amanitin

Answer 12

binds to RNA Pol II, symptoms 24 hours, death in week

Question 13

Trichostatin A

Answer 13

Histone Deactylase Inhibitor = mood stabilizer, anti-epipleptic; reactivates gene!

Question 14

Ovarian Dermoid Cyst

Answer 14

ALL FEMALE

Question 15

Hydatidiform Mole

Answer 15

All Male

Question 16

XIC

Answer 16

X Inactivation Center contains a gene, Xist, which is directly responsible for X inactivation.
Xist makes RNA PRODUCT, not protein, and coats inactive X with it.

Question 17

Prader WIlli

Answer 17

loss of paternal 15q11.12-q13.1 expression

you don’t have PWS gene turned on- underexpression of SNRPN

Question 18

Vector Must:

Answer 18

1. be capable of autonomous replication
2. must contain 1 unique restriction site
3. must have 1 gene that confers the ability to select for the vector

Question 19

Herceptin

Answer 19

Her2 inhibitor that treats Her2 breast cancer

Question 20

Tamoxifen

Answer 20

treats progesterone breast cancer

Question 21

Erlotinib and Afatinib

Answer 21

treat EGFR mutations in NSCLC

Question 22

crizotinib + certinib

Answer 22

treat ALK rearrangements in NSLCLC

Question 23

nivolumab

Answer 23

monoclonal antibody that treats squamous NSCLC