lesson one and two Flashcards
universal ancestor
bacteria: g- and g+
universal ancestor
fungi, animals and us, amoeba
sizes of organisms from smallest to largest
prion and virus (visible with electron microscope), bacteria and fungi (visible with light microscope), parasites and mammals (visible with naked eye)
how many bacteria do we have in our body?
10 trillion bacteria in the gut
E. coli genus name
Escherichia
E. coli species name
Escherichia coli
E. coli subspecies
EHEC, EPEC, ETEC
bacterial naming order
- family
- genus
- species
- subspecies
bacteria naming
Genus, species
Cocci bacteria
staphylo, strepto, diplo
bacilli bacteria
diplo, strpto, cocco
spiral bacteria
vibrio, spirillum, spirochaete
differential stains
gram stain: divides most bacteria into two groups
acid fast stain: used for mycobacterium
gram stain
based on cell wall structure: amount of peotidogylcan (structural mesh) in the bacterial cell wall
gram stain procedure steps
- fix specimen on slide using heat
- add crystal violet: penetrates into peptidoglycan
- add iodine: washes away extra CV and stains inside cells
- differentiation step: acetone or alcohol to rinse away colour
- add saffron: counter stains gram - bacteria
gram + colour
purple/blue