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MTAP - HISTOPATHOLOGIC & CYTOLOGIC TECHNIQUES MODULE > LESSON 9: MOUNTING AND LABELLING > Flashcards

LESSON 9: MOUNTING AND LABELLING Flashcards

1
Q

 after staining, the section is mounted under a coverslip using a suitable medium

A

MOUNTING

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2
Q

 uses a syrupy fluid referred to as a mountant or mounting medium

A

MOUNTING

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3
Q

Purposes of Mounting

A

 protects the specimen from physical injury
 protects the section from bleaching or deterioration due to oxidation  preserves slides for permanent keeping
 facilitates easy handling and storage

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4
Q

 sealing margins of the coverslip

A

RINGING

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5
Q

 uses Kronig cement

A

RINGING

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6
Q

 2 parts paraffin + 4-9 parts powdered colophonium resin

A

RINGING

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7
Q

Characteristics of a Good Mounting Medium

A
  1. to avoid distortion of the image, the refractive index of the mountant should be as near as possible to that of the glass which is 1.518.
  2. it should not dry quickly.
  3. It should not dissolve out or fade tissue sections
  4. It should not cause shrinkage and distortion of tissues.
  5. It should set hard, thereby producing permanent mounting of sections.
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8
Q

: for water-miscible preparations

A

Aqueous Media

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9
Q

 Has a low refractive index (1.33), moderately transparent and evaporates easily

A

Water

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10
Q

 Good for temporary mounting only

A

Water

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11
Q

 Tissue slide cannot be examined under OIO

A

Water

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12
Q

 Has a high RI (1.46) and lasts for a few minutes

A

Glycerin

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13
Q

 Provides greater visibility when mixed with water

A

Glycerin

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14
Q

(gelatin + glycerol + dist. Water + phenol crystals)

A

Glycerin Jelly

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15
Q

 Also known as Kaiser’s 1880 with a refractive index of 1.47

A

Glycerin Jelly

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16
Q

 Standard mounting medium when dehydration and clearing using xylene are not performed

A

Glycerin Jelly

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17
Q

X stain tend to fade

A

Glycerin Jelly

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18
Q

X does not set in the desired degree of hardness

A

Glycerin Jelly

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19
Q

X solidifies on storage

A

Glycerin Jelly

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20
Q

(gum Arabic + dist. Water + glycerol + sodium merthiolate)

A

Farrant’s medium

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21
Q

 Has a refractive index of 1.43

A

Farrant’s medium

22
Q

 Does not solidify on storage

A

Farrant’s medium

23
Q

X takes a longer time to harden

A

Farrant’s medium

24
Q

(gum Arabic + cane sugar/sucrose + dist. H2O, thymol)

A

Apathy’s medium

25
Q

 Has a higher refractive index of 1.52

A

Apathy’s medium

26
Q

 Used as a general purpose aqueous mountant and for methylene blue-stained nerve preparations

A

Apathy’s medium

27
Q

 Sets quite hard

A

Apathy’s medium

28
Q

 Recommended for mounting frozen sections from water

A

Brun’s fluid

29
Q

 Natural resin extracted from the Canadian tree Abus balsamea

A

Canada balsam

30
Q

 Has a refractive index of 1.524

A

Canada balsam

31
Q

 Recommended for whole mounts and thick sections

A

Canada balsam

32
Q

 Sets hard without granulation

A

Canada balsam

33
Q

X acidifies and darkens with age and upon exposure to sunlight

A

Canada balsam

34
Q

X stains are not usually preserved

A

Canada balsam

35
Q

 neutral colorless solution that dries rapidly

A

DPX (dibutylphthlate polystyrene xylene)

36
Q

 Has a refractive index of 1.532

A

DPX (dibutylphthlate polystyrene xylene)

37
Q

Recommended for small tissue sections

A

DPX (dibutylphthlate polystyrene xylene)

38
Q

 Synthetic resin mixture in xylene with a refractive index of 1.52

A

XAM

39
Q

 Available in pale, yellow or colorless solutions

A

XAM

40
Q

 Dries quickly without retraction and preserves stains well

A

XAM

41
Q

 Has a refractive index of 1.544

A

Clarite

42
Q

 Usually diluted to 60% with xylene

A

Clarite

43
Q

For temporary mounting

A

Water 1.33

44
Q

For moist sections

A

Glycerin 1.46

45
Q

Standard mountant when dehydration and clearing with xylene are not performed

A

Glycerin jelly 1.47

46
Q

For temporary mounting

A

Farrant’s medium 1.43

47
Q

For general purpose mounting and methylene blue-stained nerve preparations

A

Apathy’s medium 1.52

48
Q

For frozen sections

A

Brun’s fluid

49
Q

For whole mounts and thick sections

A

Canada Balsam 1.524

50
Q

For small tissue sections

A

DPX 1.532

51
Q

For permanent mounting

A

XAM 1.52

52
Q

For permanent mounting

A

Clarite 1.544

MTAP - HISTOPATHOLOGIC & CYTOLOGIC TECHNIQUES MODULE (9 decks)

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