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MTAP - HISTOPATHOLOGIC & CYTOLOGIC TECHNIQUES MODULE > LESSON 1: INTRODUCTION TO TISSUE PROCESSING > Flashcards

LESSON 1: INTRODUCTION TO TISSUE PROCESSING Flashcards

1
Q

✓ preparation, processing and staining of tissue sections of tissue sections for microscopic study to be interpreted by the pathologist

A

HISTOTECHNIQUES

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2
Q

✓ study of disease at the tissue level

A

HISTOPATHOLOGY

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3
Q

: examined to determine the cause of death

A
  1. Autopsy Materials
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4
Q

: otherwise referred to as surgical or biopsy materials; examined to provide a diagnosis

A
  1. Surgical Materials
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5
Q

: removal of cells from the area of abnormality

A

A. FINE NEEDLE ASPIRATION

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6
Q

 considered as the simplest and least invasive method of collecting biopsy specimens

A

A. FINE NEEDLE ASPIRATION

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7
Q

 method of collection for fluid-containing tumors

A

A. FINE NEEDLE ASPIRATION

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8
Q

: removal of cells and small amount of surrounding tissue

A

B. CORE NEEDLE BIOSY

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9
Q

: removal of cells with more surrounding tissue

A

C. INCISIONAL BIOPSY

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10
Q

: removal of the entire area in question

A

D. EXCISIONAL BIOPSY

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11
Q

 Ensure complete removal of the lesion

A

D. EXCISIONAL BIOPSY

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12
Q

 Confirm that the diagnosis is correct

A

D. EXCISIONAL BIOPSY

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13
Q

: removal of 3 to 4 mm cylindrical core of tissue samples

A

E. PUNCH BIOPSY

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14
Q

 small: 2mm; large: 4mm

A

E. PUNCH BIOPSY

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15
Q

 lesion should be at the center

A

E. PUNCH BIOPSY

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16
Q

: removal of small fragments of tissue from a surface

A

F. SHAVE BIOPSY

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17
Q

: removal of tissue or growths from body cavities

A

G. CURETTINGS

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18
Q

STORAGE

A
  1. Specimen: 1 month to 1 year
  2. Tissue Blocks: 3 to 10 years
  3. Slides: Indefinite
  4. Records (request and result forms): Permanent
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19
Q

FACTORS TO BE CONSIDERED IN CHOOSING A METHOD

A
  1. Structural and chemical components to be studied
  2. Nature and amount of sample to be evaluated
  3. The need to provide an immediate diagnosis
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20
Q

 No fixative required

A

FRESH TISSUE EXAMINATION

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21
Q

 Examined using a Brightfield or Phase-Contrast microscope

A

FRESH TISSUE EXAMINATION

22
Q

 Stained with supravital or differential dyes

A

FRESH TISSUE EXAMINATION

23
Q

 Observation of physiologic processes or protoplasmic activities (motion, mitosis, phagocytosis and pinocytosis)

A

FRESH TISSUE EXAMINATION

24
Q

 Relatively simple and easy to perform

A

FRESH TISSUE EXAMINATION

25
Q

 Limited use

A

FRESH TISSUE EXAMINATION

26
Q

 Dissection or separation of tissue components in NSS or Ringer’s solution

A

TEASING

27
Q

 Examined as stained or unstained

A

TEASING

28
Q

 Anatomical relationship is destroyed

A

TEASING

29
Q

 Tissue (<1mm) is sandwiched between two slides

A

SQUASH

30
Q

 Stain is applied on one side of the slide and allowed to spread via capillary action

A

SQUASH

31
Q

 for cytological studies, especially for the diagnosis of cancer

A

SMEARING

32
Q

 for sections or sediments

A

SMEARING

33
Q

 performed using a wire loop, applicator stick or another slide

A

SMEARING

34
Q

 Uniform distribution in a direct or zigzag manner

A

 Streaking

35
Q

 Thick or mucoid specimens

A

 Spreading

36
Q

 Teasing on a slide

A

 Spreading

37
Q

 Maintains intercellular relationship

A

 Spreading

38
Q

 For the preparation of blood and bone marrow smears

A

 Pull-Apart

39
Q

 One side of a slide is allowed to touch a surface of the sample

A

 Touch Preparation

40
Q

 Intercellular relationship is maintained

A

 Touch Preparation

41
Q

 Prepared using freezing microtome or cryostat

A

FROZEN SECTION

42
Q

 For rapid diagnosis

A

FROZEN SECTION

43
Q

Initial Steps in Tissue Processing

A

A. Specimen Accessioning/Identification

B. Gross Examination and Sampling

C. Tissue Processing

44
Q

C. Tissue Processing:

A

 Fixation
 Dehydration
 Clearing
 Infiltration
 Embedding
 Sectioning (+ Floating, Fishing-out, Drying)
 Staining
 Mounting
 Labelling

45
Q

C. Tissue Processing

A
46
Q

 Used to locate the presence and position of mineral elements in the tissue

A
  1. MICROINCINERATION
47
Q

 Two duplicate sections of alcohol-fixed tissues

A
  1. MICROINCINERATION
48
Q

 Injection of radioactive isotopes into organs

A
  1. AUTORADIOGRAPHY
49
Q
  1. AUTORADIOGRAPHY:
A

Fix>Section>Mount + Photographic Emulsion (Ag Halide)

50
Q

 Determines the relationship and location of the isotopes and cells to be studied

A

Stain

51
Q

 Provides qualitative and quantitative information

A

Stain

MTAP - HISTOPATHOLOGIC & CYTOLOGIC TECHNIQUES MODULE (9 decks)

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