Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

MTAP - HISTOPATHOLOGIC & CYTOLOGIC TECHNIQUES MODULE > LESSON 1: INTRODUCTION TO TISSUE PROCESSING > Flashcards

LESSON 1: INTRODUCTION TO TISSUE PROCESSING Flashcards

1
Q

✓ preparation, processing and staining of tissue sections of tissue sections for microscopic study to be interpreted by the pathologist

A

HISTOTECHNIQUES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

✓ study of disease at the tissue level

A

HISTOPATHOLOGY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

: examined to determine the cause of death

A
  1. Autopsy Materials
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

: otherwise referred to as surgical or biopsy materials; examined to provide a diagnosis

A
  1. Surgical Materials
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

: removal of cells from the area of abnormality

A

A. FINE NEEDLE ASPIRATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

 considered as the simplest and least invasive method of collecting biopsy specimens

A

A. FINE NEEDLE ASPIRATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

 method of collection for fluid-containing tumors

A

A. FINE NEEDLE ASPIRATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

: removal of cells and small amount of surrounding tissue

A

B. CORE NEEDLE BIOSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

: removal of cells with more surrounding tissue

A

C. INCISIONAL BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

: removal of the entire area in question

A

D. EXCISIONAL BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

 Ensure complete removal of the lesion

A

D. EXCISIONAL BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

 Confirm that the diagnosis is correct

A

D. EXCISIONAL BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

: removal of 3 to 4 mm cylindrical core of tissue samples

A

E. PUNCH BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

 small: 2mm; large: 4mm

A

E. PUNCH BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

 lesion should be at the center

A

E. PUNCH BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

: removal of small fragments of tissue from a surface

A

F. SHAVE BIOPSY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

: removal of tissue or growths from body cavities

A

G. CURETTINGS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

STORAGE

A
  1. Specimen: 1 month to 1 year
  2. Tissue Blocks: 3 to 10 years
  3. Slides: Indefinite
  4. Records (request and result forms): Permanent
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

FACTORS TO BE CONSIDERED IN CHOOSING A METHOD

A
  1. Structural and chemical components to be studied
  2. Nature and amount of sample to be evaluated
  3. The need to provide an immediate diagnosis
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

 No fixative required

A

FRESH TISSUE EXAMINATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

 Examined using a Brightfield or Phase-Contrast microscope

A

FRESH TISSUE EXAMINATION

22
Q

 Stained with supravital or differential dyes

A

FRESH TISSUE EXAMINATION

23
Q

 Observation of physiologic processes or protoplasmic activities (motion, mitosis, phagocytosis and pinocytosis)

A

FRESH TISSUE EXAMINATION

24
Q

 Relatively simple and easy to perform

A

FRESH TISSUE EXAMINATION

25
 Limited use
FRESH TISSUE EXAMINATION
26
 Dissection or separation of tissue components in NSS or Ringer’s solution
TEASING
27
 Examined as stained or unstained
TEASING
28
 Anatomical relationship is destroyed
TEASING
29
 Tissue (<1mm) is sandwiched between two slides
SQUASH
30
 Stain is applied on one side of the slide and allowed to spread via capillary action
SQUASH
31
 for cytological studies, especially for the diagnosis of cancer
SMEARING
32
 for sections or sediments
SMEARING
33
 performed using a wire loop, applicator stick or another slide
SMEARING
34
 Uniform distribution in a direct or zigzag manner
 Streaking
35
 Thick or mucoid specimens
 Spreading
36
 Teasing on a slide
 Spreading
37
 Maintains intercellular relationship
 Spreading
38
 For the preparation of blood and bone marrow smears
 Pull-Apart
39
 One side of a slide is allowed to touch a surface of the sample
 Touch Preparation
40
 Intercellular relationship is maintained
 Touch Preparation
41
 Prepared using freezing microtome or cryostat
FROZEN SECTION
42
 For rapid diagnosis
FROZEN SECTION
43
Initial Steps in Tissue Processing
A. Specimen Accessioning/Identification B. Gross Examination and Sampling C. Tissue Processing
44
C. Tissue Processing:
 Fixation  Dehydration  Clearing  Infiltration  Embedding  Sectioning (+ Floating, Fishing-out, Drying)  Staining  Mounting  Labelling
45
C. Tissue Processing
46
 Used to locate the presence and position of mineral elements in the tissue
1. MICROINCINERATION
47
 Two duplicate sections of alcohol-fixed tissues
1. MICROINCINERATION
48
 Injection of radioactive isotopes into organs
2. AUTORADIOGRAPHY
49
2. AUTORADIOGRAPHY:
Fix>Section>Mount + Photographic Emulsion (Ag Halide)
50
 Determines the relationship and location of the isotopes and cells to be studied
Stain
51
 Provides qualitative and quantitative information
Stain

MTAP - HISTOPATHOLOGIC & CYTOLOGIC TECHNIQUES MODULE (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions