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Histopathology Laboratory > Lesson 6: Infiltration and Embedding > Flashcards

Lesson 6: Infiltration and Embedding Flashcards

1
Q

Process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill out the tissue cavities

A

Infiltration

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2
Q

A good infiltrating medium is ideally molten between ___ to ___

A

30C to 60C

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2
Q

A good infiltrating medium should have a melting point of below ___ in the pure state

A

Below 60C

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2
Q

2 Types of Infiltration Media

A

Water Miscible
Water Immiscible

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3
Q

Overheated paraffin makes the specimen _______

A

brittle

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3
Q

3 Types of Water Miscible Media

A

Gelatin
Carbowax
Agar

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4
Q

2 Types of Water Miscible Media

A

Paraffin Wax
Paraplast

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4
Q

Also known as blocking/casting

A

Embedding

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5
Q

True or False

Paraffin processing is recommended for fatty tissues

A

False, it is not recommended

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5
Q

Types of Embedding Mold (4)

A

Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Ring & Base Mold
Disposable Embedding Mold

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6
Q

This will promote retention of the clearing agent

A

Inadequate impregnation

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6
Q

Excessive tissue shrinkage and hardening is caused by ____________ impregnation

A

Prolonged

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7
Q

3 Types of Disposable Embedding Mold

A

Peel-away
Plastic Ice Tray
Paper Boat

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7
Q

It is a mold that consists of 2-L shaped strips of heavy brass or metal

Mold is adjustable to make different size of blocks

A

Leuckhart Mold

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8
Q

It is a mold made up of a series of interlocking plates resting on a flat metal base

Advantage: Embedding more specimens at a time

A

Compound Embedding Unit

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9
Q

It is a mold that consists of a special stainless steel base mold fitted with a plastic embedding ring, which serves as the block holder during cutting

A

Plastic Embedding Ring

10
Q

A disposable thin plastic embedding mold. They are simply peeled off one at a time, as soon as the wax has solidified, giving perfect even block without trimming

A

Peel Away

11
Q

May be recommended by busy routine laboratories. Each compartment may be utilized for embedding “one tissue block”

A

Plastic Ice Trays

12
Q

A type of disposable mold:

Oldest method
Advantage of being cheap and easy to make

A

Paper Boat Method

13
Q

Melting Point of paraffin wax

A

40-60C

14
Q

For tropical countries, hard wax having a melting point of __ to __ is suitable

A

58-60C

14
Q

A synthetic wax substitute similar to paraplast with a melting point of 56-58C

A

Embeddol

14
Q

Most commonly used tissue embedding medium

A

Paraffin Wax

15
Q

Melting Point of Paraplast

A

56-57C

15
Q

This is a mixture of purified paraffin and plastic

A

Paraplast

15
Q

Easy to make the serial sections of 4 microns due to its elasticity

A

Paraplast

16
Q

It is less brittle and less compressible than paraplast

A

Embeddol

16
Q

A semi-synthetic wax recommended for embedding “eyes”

A

Bioloid

17
Q

A product of paraffin, containing rubber, with the same property as a paraplast

A

Tissue Mat

17
Q

Harder than paraffin but has a low melting point of 46-48C

A

Ester Wax

18
Q

A mixture of paraffin and rubber

A

Tissue Mate

19
Q

It is rarely used except when dehydration is to be avoided and when tissues are subjected to histochemical and enzyme studies

A

Gelatin

20
Q

Used for delicate specimens and frozen tissue sections
Water-soluble
Low melting point
Tissues should not be more than 2-3 mm thick

A

Gelatin

21
Q

Gelatin contains this solution that serves to prevent the “growth of molds”

A

1% phenol

21
Q

A purified form of nitrocellulose, soluble in many solvents

A

Celloidin

22
Q

This is suitable for specimens with large hollow cavities
Used for hard and dense tissues
Recommended for processing “Neurological Tissues”

A

Celloidin

23
Q

“Light loose texture materials” are best stirred up while the paraffin wax is ______, if not followed it may not aggregate in the center of the mold

A

warm

Histopathology Laboratory (5 decks)

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