Lesson 6 (Biosafety and Biosecurity) Flashcards
What do you call the pathogens that are commonly acquired
Laboratory Acquired Infections (LAI)
Protecting people from dangerous pathogens
Biosafety
Protecting pathogens from dangerous people
Biosecurity
When and where did the history of laboratory biosafety took place
North America and West Europe (1943)
The first person who created the Biological Development/ Biological Weapons Program
Franklin Roosevelt
When did he create the Biological Weapon Program
during cold war
(blank) focused only on the biological usage or microorganism that are against the (blank)
Biological Program; Soviet Union
The first scientific director in 1943 of Camp Detrick
Ira L. Baldwin
The center of the US Biological Program and also the designated installation for Biological Research and Development
Camp Detrick
new name for Camp Detrick
Fort Detrick
Designed multiplications for Biosafety at Camp Detrick
Newell Johnson
The meetings of Johnson eventually lead to (blank)
The American Biological Safety Association (ABSA)
He terminated the Biological Program that created by Roosevelt + (what year)
Richard Nixon in 1969
First person who invented the Mechanical Pipettor
Arnold Wedum
Another invention of Arnold Wedum
Ventilation Cabinet to prevent infection against MTB or Macrobacterium Tuberculosis
Defined as any biological or chemical substance that is dangerous to
human, animals or the environment
Biohazard
Examples of biohazard
body fluids, human tissue and blood, and recombinant DNA
Used universally to report Harmful substances that are particularly
dangerous for living beings
Biohazard symbol
Who created the Biohazard symbol (year)
Charles Baldwin (1966)
Agents that are not associated with disease in healthy adult
humans. The low individual and community risk.
RG 1
Agents that are associated with human disease which is
rarely serious and for which preventive or therapeutic inventions are
often available. Moderate individual and low community risk.
RG 2
Agents that are associated with serious or lethal human
disease for which preventive or therapeutic or interventions may be
available. High individual risk but low community risk.
RG 3
Agents that are likely to cause serios or lethal human disease
for which preventive or therapeutic inventions are not usually
available. High individual risk and high transmissible to an
individual.
RG 4
Suitable for work involving variable microorganism that are defined
and well characterized strains known not to cause disease in human; appropriate among undergraduate and secondary educational
training and teaching laboratories that require basic lab safety
practices; no containment needed
Biosafety lvl 1
Containment for Biosafety lvl 1
no containment needed
Ex. for biosafety lvl 1
Bacillus subtilis, Naegleria gruberi, Infectious canine hepatitis
virus
Risk Group 1 Organism
E coli K-21
Transgenic Plants
Plasmids
Fungi
Molds
Yeast
For lab that deals with indigenous MODERATE RISK agents; lab consequently observing good microbial techniques; when handling human blood, body fluid, tissues where there is
uncertain presence of infections agents; handwashing sinks and waste decontamination facility
Biosafety lvl 2
Containment for Biosafety lvl 2
biosafety cabinets
Ex. for biosafety lvl 2
Hepa B, HIV Salmonella, Toxoplasma
Risk Group 2 Organism
Human or Primate Cells
Herpes simplex Virus
Replication Incompetent Attended Human
Immunodeficiency Virus
Patient specimens
Primary and secondary barriers in the protections of the personnel
from infectious aerosol exposure; indigenous or toxic agents with potential for respiratory transmission that may cause serious and lethal infections
Biosafety lvl 3
Ex. for biosafety lvl 3
MTB, St. louis Encephalitis Virus
Risk Group 3 Organisms
Human Immunodeficiency Virus
Mycobacterium Tuberculosis
Coxiella Bumetii
Dangerous and exotic agents that pose high individual risk of
life-threatening disease that may be transmitted via aerosol
No vaccine or treatment
Separate building or completely isolated zone
Builds on BSL-3 / ABSL-3 practices
Biosafety lvl 4
Containment for Biosafety lvl 4
BSL-3 + Class III Biosafety Cabinet
Risk Group 4 Organisms
Lassa Fever Virus
Ebola Hemorrhagic Fever Virus
Marburg Virus
Herpes B Virus
meaning of HEPA filtered air
High Efficiency Particulate Air
Provides protection foe the user and surrounding
environment, but no protection for the sample being
manipulated
Biosafety Cabinets Class 1
provides protections for the user, environment
and sample, and divides into four types: A1, A2, B3 and
B2. The main difference are their minimum inflow
velocities and exhaust system
Biosafety Cabinet Class 2
Also known as gloves boxes
Provides maximum protections; he enclosure is gas
light and all materials enter leave through a dunk tank
or double door autoclave
Biosafety Cabinet Class 3
