LESSON#3: THE PROPER TECHNIQUE OF MOUNTING STAINED TISSUE SECTIONS

Question 1

when should mounting be done?

Answer 1

immediately after staining

Question 2

2 methods for mounting stained tissue sections

Answer 2

slide method
coverslip method

Question 3

method req the mounting media to be placed first on TOP OF THE TISSUE sample on the GLASS slide –> coverslip

Answer 3

SLIDE METHOD

Question 4

method req the mounting media to be placed first on the COVERSLIP –> glass slide (w tissue sample) on surface

Answer 4

coverslip method

Question 5

effects of oxidation on stained tissues

Answer 5

bleaching and deterioration

Question 6

to avoid viewing artifacts, the slide and coverslip must be free of ?

Answer 6

optical distortions

Question 7

it is used to adhere the coverslip to the slide

Answer 7

mounting medium

Question 8

water carried over to the mounting stage will up as –

Answer 8

bubbles or vacuole like structures

Question 9

2 types of mounting media

Answer 9

aqueous media
resinous media

Question 10

usually used for mounting material which is unstained

Answer 10

aqueous media

Question 11

used for mounting sections from DISTILLED WATER when the stains would be decolorized or removed by ALCOHOL AND XYLENE

Answer 11

AQUEOUS MED

Question 12

stain that tend to diffuse into the medium after mounting

Answer 12

methyl violet

Question 13

how to prevent methyl violet from diffuding into the medium after mounting

Answer 13

using HIGHMAN’S MEDIUM

Question 14

bacteriostatic agents - to prevent grotwh of fungi

Answer 14

phenol
crystal of thymol
sodium merthiolate

Question 15

commonly used aqueous mounting media

Answer 15

(FHAG)

farrant’s medium
highman’s medium
apathy’s medium
glycerine jelly

Question 16

medium for mounting sections for FLUORESCENT MICROSCOPY

Answer 16

apathy’s medium (1.52)

Question 17

reco for fat stains

Answer 17

farrant’s medium (1.43)

Question 18

routine mordant for fat stains

Answer 18

glycerine jelly (1.47)

Question 19

reco for metachromatic dyes like METHYL VIOLET

Answer 19

Highmann’s medium (1.52)

Question 20

allow the mounting of tissues DIRECTLY FROM THE STAINING PROCEDURE

Answer 20

aqueous mounting media

Question 21

tyoe of mounting media: water solubility of some stains allow them to bleed or fade

Answer 21

aqueous mounting media

Question 22

type of media: tissue section must first be dehydrated and cleared

Answer 22

resinous mounting media

Question 23

most common mounting media used in routine processing

Answer 23

resinous media

Question 24

resinous media are made up of NATURAL or SYNTHETIC resins dissolved in ?

Answer 24

benzene
toluene
xylene

Question 25

used as a 60% natural resin by weight in xylene

Answer 25

canada balsam (1.52)

Question 26

in canada balsam: - fairly well preserved

Answer 26

h&e

Question 27

in canada balsam: - tend to fade

Answer 27

basic aniline dyes

Question 28

in canada balsam: slowly bleached

Answer 28

prussian blue

Question 29

slides take a few months to dry

Answer 29

canada balsam

Question 30

most commonly used resinous media

Answer 30

d.p.x (1.52)

Question 31

in d.p.x ? dissolved in xylene as a 20% solution

Answer 31

polystyrene resin

Question 32

? may be done after mounting the cover slip

Answer 32

permanent sealing or ringing

Question 33

coverslip may be ringed with the used of - for storage

Answer 33

clear nail polish

Question 34

refractive index of glass

Answer 34

1.518

Question 35

apply - drop of mounting medium upon tissue section

Answer 35

1 drop

Question 36

angle of coverslip

Answer 36

45