LESSON #1: PROPER TECHNIQUE OF SECTIONING TISSUE SAMPLES

Question 1

microtome used in the lab

Answer 1

Leica RM2125 RTS

Question 2

sections produced by the Leica RM2125 RTS

Answer 2

0-60 um

Question 3

initial sectioning: used at the very beginning of the secioning procedure to clear the tissue block (2 dots)

Answer 3

2.5 um

Question 4

• dots used in todet to TRIM the tissue block enough to straighten it

Answer 4

2

Question 5

final sectioning: used at the end of the sectioning procedure for the final cutting = final ribbons (1 dot)

Answer 5

10 um

Question 6

temp of the floatation bath

Answer 6

5-10C below the melting point of the paraffin wax

Question 7

utilized in order to affix the tissues to the glass slides prior to staining and viewing under the microscope

Answer 7

adhesives

Question 8

most commonly used adhesive

Answer 8

Meyer’s Egg Albumin (1:1 concentration)

Question 9

Meyer’s Egg Albumin composition

Answer 9

1ml egg white
1ml glycerine

Question 10

added in the adhesive to PREVENT MOLDS; allow the slide to LAST FOR 3-4 MONTHS

Answer 10

phenol

Question 11

also known as sectioning of cutting

Answer 11

microtomy

Question 12

process where tissues are cut into uniformly thin slices

Answer 12

microtomy

Question 13

paraffin embedded tissues are sectioned from - to facilitate microscopic studies

Answer 13

4-8 microns

Question 14

basic instrument used to facilitate microscopic studies

Answer 14

microtome

Question 15

microtome knives: 4 types

Answer 15

standard thick metal
thin disposable blades
glass knives
diamond knives

Question 16

expensive knives; allows sharpening to your liking

Answer 16

standard thick metal knives

Question 17

do the same job as the standard thick and probably better for a much lesser cost

Answer 17

thin disposable blades

Question 18

these tissue sections are sectioned with GLASS OR DIAMOND KNIFE

Answer 18

embedded in plastic material: METHACRYLATE, ARALDITE, EPON

Question 19

can section down to about 1 micron

Answer 19

glass knife

Question 20

– microscopy thin sections are preferred sectioned to about 1/4 of a micron

Answer 20

electron

Question 21

for sections of about 1/4 of a micron

Answer 21

diamond knife

Question 22

artists of the laboratory

Answer 22

histotechnologists

Question 23

common artifacts

Answer 23

tearing, ripping, holes, folding

Question 24

helps REMOVE WRINKLES in sections

Answer 24

when sections are floated on a WARM WATER BATH

Question 25

advantageous or not? excessive bending of section at the cut line

Answer 25

not advantageous

Question 26

prevents contact between the knife facet and the face of the block

Answer 26

clearance angle

Question 27

angle between the two facets that form the cutting edge

Answer 27

facet or bevel angle

Question 28

for ROUTINE, knives and disposable blades are made with a facet angle of approx.

Answer 28

35 degrees

Question 29

clearance angle for ROTARY MICROTOME KNIVES and BLADE HOLDERS

Answer 29

0-15 degrees is recommended