Lesson 2- Manipulating DNA

Question 1

How do scientists make changes to DNA?

Scientists use different techniques to:

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•
•
•

Answer 1

• Extract DNA from cells
• Cut DNA to smaller pieces
• Identify the sequence of bases in a DNA molecule
• Make unlimited copies of DNA

Question 2

(Blank) (blank), biologists make changes in the DNA code of a living organism.

Answer 2

Genetic engineering

Question 3

DNA Extraction

DNA can be extracted from most cells by a simple (blank) (blank)

Answer 3

medical procedure.

Question 4

DNA Extraction
The cells are (blank) and the DNA is separated from the other cell parts

Answer 4

opened

Question 5

Cutting DNA

Most DNA molecules are too large to be analyzed, so biologists cut them into smaller fragments using (blank)

Answer 5

restriction enzymes

Question 6

Each restriction enzyme cuts DNA at a (sentence blank)

Answer 6

specific sequence of nucleotides.

Question 7

Separating DNA

In (blank) (blank), DNA fragments are placed at one end of a porous gel, and an electric voltage applied to the gel.

Answer 7

gel electrophoresis

Question 8

Gel Electrophoresis
First (blank) (blank) cut DNA into fragments

Answer 8

restriction enzymes

Question 9

Gel Electrophoresis

An (blank) (blank) is applied to the gel.

Answer 9

electric voltage

Question 10

Gel Electrophoresis
The smaller the DNA
fragment the (blank) and (blank) it will move across the gel

Answer 10

faster and farther

Question 11

Making Copies

(Blank) (blank) (blank) (blank) is a technique that allows biologists to make copies of genes

Answer 11

Polymerase chain reaction (PCR) is

Question 12

Making Copies
Small amounts of DNA can be multiplied making it (blank) to (blank).

Answer 12

easier to analyze.

Question 13

Making Copies
Made possible by an enzyme found in a bacterium living in hot springs in (blank) (blank) (blank) (blank)

Answer 13

Yellow Stone National Park.