Lesson 2

Question 1

are growth of microorganisms on culture medium

Answer 1

culture

Question 2

is the media containing the required nutrients for bacterial growth

Answer 2

culture medium

Question 3

Microbes that are introduced into a culture medium to initiate growth

Answer 3

inoculum

Question 4

the needs are complex; exceptional media components must be used for growth

Answer 4

fastidious

Question 5

the nutritional needs are relatively basic

Answer 5

nonfastidious

Question 6

types of culture:

Answer 6

pure, mix and stock

Question 7

one genus

Answer 7

pure culture

Question 8

more than 2 genus and species

Answer 8

mix culture

Question 9

for academic/industrial purposes

Answer 9

stock culture

Question 10

when their contents are allowed to solidify with the tube held at an angle so that a large surface area for
growth is available

Answer 10

agar slant

Question 11

when the agar solidifies in a vertical tube
Broth

Answer 11

agar deep

Question 12

contains nutrients for bacterial growth

Answer 12

COMPLEX MEDIA or NON-SYNTHETIC MEDIA

Question 13

Hydrolyzed product of animal and plant proteins: Free
amino acids, peptides and proteases(large sized
peptides).

Answer 13

peptone

Question 14

source of carbon and energy assist in the
differentiation of bacteria

Answer 14

carbohydrates

Question 15

supply amino acids, vitamin and mineral salt

Answer 15

meat extract

Question 16

a polysaccharide derived from marine red algae

Answer 16

agar

Question 17

the precise chemical composition and amount of all components are
known

Answer 17

syntethic or defined medium

Question 18

recommended for organisms that cannot grow in cell free media

Answer 18

tissue

Question 19

Allows the growth of aerobes, anaerobes and facultative anaerobes

Answer 19

liquid medium

Question 20

Uses as an enrichment media
and as motility media

Answer 20

semi-solid medium

Question 21

Routinely used in the laboratory without added supplement

Answer 21

Basic/Supportive /General purpose media

Question 22

Used to propagate the growth of certain group of organisms

Answer 22

enrichment media

Question 23

Formulated to provide distinct colonial appearances based on certain biochemical reaction such as lactose fermentation

Answer 23

differential media

Question 24

It is incorporated with antibiotics, dyes or chemicals to inhibit the growth of other organisms while promoting the
growth of the desired organism

Answer 24

selective media

Question 25

Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria
when specimens cannot be cultured soon after collection

Answer 25

transport media

Question 26

incubation for fungi

Answer 26

28 to 30 degrees celsius

Question 27

incubation for most bacteria, viruses, and acid-fast bacillus

Answer 27

35 to 37 degrees celsius

Question 28

grow in ambient air, which contains 21% oxygen and a small amount (0.03%) of carbon dioxide

Answer 28

aerobes

Question 29

anaerobe jars, bags, or chambers is composed of 5% to 10% hydrogen, 5% to 10% CO2, 80% to 90% nitrogen, and 0% oxyge

Answer 29

anaerobes