Lesson 2 Flashcards
are growth of microorganisms on culture medium
culture
is the media containing the required nutrients for bacterial growth
culture medium
Microbes that are introduced into a culture medium to initiate growth
inoculum
the needs are complex; exceptional media components must be used for growth
fastidious
the nutritional needs are relatively basic
nonfastidious
types of culture:
pure, mix and stock
one genus
pure culture
more than 2 genus and species
mix culture
for academic/industrial purposes
stock culture
when their contents are allowed to solidify with the tube held at an angle so that a large surface area for
growth is available
agar slant
when the agar solidifies in a vertical tube
Broth
agar deep
contains nutrients for bacterial growth
COMPLEX MEDIA or NON-SYNTHETIC MEDIA
Hydrolyzed product of animal and plant proteins: Free
amino acids, peptides and proteases(large sized
peptides).
peptone
source of carbon and energy assist in the
differentiation of bacteria
carbohydrates
supply amino acids, vitamin and mineral salt
meat extract
a polysaccharide derived from marine red algae
agar
the precise chemical composition and amount of all components are
known
syntethic or defined medium
recommended for organisms that cannot grow in cell free media
tissue
Allows the growth of aerobes, anaerobes and facultative anaerobes
liquid medium
Uses as an enrichment media
and as motility media
semi-solid medium
Routinely used in the laboratory without added supplement
Basic/Supportive /General purpose media
Used to propagate the growth of certain group of organisms
enrichment media
Formulated to provide distinct colonial appearances based on certain biochemical reaction such as lactose fermentation
differential media
It is incorporated with antibiotics, dyes or chemicals to inhibit the growth of other organisms while promoting the
growth of the desired organism
selective media
Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria
when specimens cannot be cultured soon after collection
transport media
incubation for fungi
28 to 30 degrees celsius
incubation for most bacteria, viruses, and acid-fast bacillus
35 to 37 degrees celsius
grow in ambient air, which contains 21% oxygen and a small amount (0.03%) of carbon dioxide
aerobes
anaerobe jars, bags, or chambers is composed of 5% to 10% hydrogen, 5% to 10% CO2, 80% to 90% nitrogen, and 0% oxyge
anaerobes
