Lesson 1

Question 1

kills all microorganisms (including spores & viruses)

Answer 1

sterilization

Question 2

inactivation or inhibition of microorganisms

Answer 2

disinfection

Question 3

physical methods of control

Answer 3

heat, filtration & radiation

Question 4

the most common method used

Answer 4

heat

Question 5

causes irreversible denaturation of enzymes and structural proteins

Answer 5

moist heat sterilization

Question 6

denatures and coagulates protein

Answer 6

moist heat sterilization

Question 7

kills negative forms of bacterial pathogens, almost all viruses

Answer 7

boiling

Question 8

disadvantage of moist heat sterilization

Answer 8

some bacterial endospores can resist boiling for more than 20 hours

Question 9

most dependable method

Answer 9

steam under pressure

Question 10

kill all microorganisms (but not prions)

Answer 10

steam under pressure

Question 11

biological indicator for autoclave

Answer 11

stearothermophilus spores

Question 12

used to sterilized milks, dairy products and alcohol

Answer 12

pasteurization

Question 13

sterilization without water

Answer 13

dry heat sterilization

Question 14

simplest method of dry heat sterilization

Answer 14

flaming

Question 15

most common method of treating infectious waste and infected laboratory animal

Answer 15

incineration

Question 16

use of hot air oven

Answer 16

hot air sterilization

Question 17

sterilization of disposable such as syringes, gloves and catheters

Answer 17

ionizing

Question 18

damages dna

Answer 18

nonionizing

Question 19

used to sterilize liquids that are thermolabile

Answer 19

filtration

Question 20

accomplished through the use of thin membrane filters composed of plastic polymers or cellulose esters
containing pores of a certain size

Answer 20

liquid

Question 21

accomplished with the use of HEPA filters

Answer 21

air

Question 22

able to remove microorganisms larger than 0.3 µm
: used in laboratory hoods and in rooms of immunocompromised patients
: employed in biosafety cabinets

Answer 22

HEPA filters

Question 23

chemical agents used for sterilization

Answer 23

chemosterilizers

Question 24

considered to be important pioneers for the promotion of asepsis

Answer 24

Ignaz Semmelweis and Joseph Lister

Question 25

Substance designed to be used on inanimate objects to kill or destroy disease-producing microorganisms, including
spores in some cases

Answer 25

disinfectants

Question 26

sustance that inhibits, destroys, or reduces the bacterial load of living tissues or microorganisms

Answer 26

antiseptics

Question 27

it precipitates bacterial protein

Answer 27

bactericidal

Question 28

directly kills bacteria

Answer 28

bactericidal

Question 29

antimicrobial that inhibits bacterial growth but does not kill the bacteria

Answer 29

bacteriostatic

Question 30

Biological indicator of oven

Answer 30

basillus subtilis var niger