LESSON 1: LAB SAFETY Flashcards
consist of burns to the sclera, conjunctiva, cornea, and eyelids.
Ocular burns
3rd highest prevalence in the world
Primary tuberculosis
Any microbiological entity (cellular or non), that is naturally occurring or engineered, which is capable of replication or transferring genetic material that may affect other organisms
Viruses, bacteria, fungi, parasites, prions
Biological agents
Any object/specimen comprised or containing/may contain biological ag or their harmful products
Biological material
Containment principles, technologies, and practices implemented to prevent UNINTENTIONAL exposure to or release of pathogens and toxins
Biosafety
Institutional and personal security measures designed to prevent the loss, theft, misuse, diversion or INTENTIONAL release of pathogens and toxins
Biosecurity
Physical enclosure for pathogens by isolation in environmentally and biologically secure cabinets or rooms
Biocontainment
Can be primary or secondary
Biocontainment
Control hazard at the source
Primary
Provides personnel and environmental protection
but not product
Unsterilized room air is drawn over the work surface
Class I BSC
Has four types (Al, A2, B1, and B2) that differ
depending on the ratio of air exhausted
Has inward airflow as well as downward HEPA
filtered laminar airflow over the work surface
Class II BSC
● Highest level of personnel protection
● Totally enclosed with glove ports
● Airflow is maintained by a dedicated exhaust
system exterior to the cabinet to keep the cabinet
under negative pressure
Class III BSC
Structure surrounding primary containment
Secondary
● Well characterized, non-pathogenic organisms or
agents
● OPEN bench - NO containment
● Use good lab practices, waste disposal, and aseptic
techniques
BSL 1
● Builds upon BSL-1
● Agents of moderate hazard to personnel or
environment (non- respiratory, non-lethal)
● Restricted access
BSL 2
● High containment
● Agents of high hazard to personnel or environment
● Agents that may cause serious or potentially lethal
disease through the inhalation route of exposure
BSL 3
● MAXIMUM containment
● Dangerous and exotic agents that pose high
individual risk of aerosol transmitted laboratory
infections
● Positive pressure “moonsuits”
BSL 4
CLASSIFICATION OF INFECTIVE MICROORGANISMS
BY RISK GROUP
No or LOW individual/community risk
1
CLASSIFICATION OF INFECTIVE MICROORGANISMS
BY RISK GROUP
MODERATE individual risk, LOW
community risk
2
CLASSIFICATION OF INFECTIVE MICROORGANISMS
BY RISK GROUP
HIGH individual risk, LOW community risk
3
CLASSIFICATION OF INFECTIVE MICROORGANISMS
BY RISK GROUP
HIGH individual / community risk
4
INFECTIOUS AGENTS AND THEIR RISK GROUPS
Japanese encephalitis
RISK GROUP 2 - Virus
INFECTIOUS AGENTS AND THEIR RISK GROUPS
Toxoplasmosis
RISK GROUP 2 - Parasite
INFECTIOUS AGENTS AND THEIR RISK GROUPS
Novel human coronavirus (SARS)
RISK GROUP 3 - virus
INFECTIOUS AGENTS AND THEIR RISK GROUPS
Melioidosis
RISK GROUP 3 - bacteria
INFECTIOUS AGENTS AND THEIR RISK GROUPS
Crimean congo hemorrhagic fever
RISK GROUP 4 - Virus
INFECTIOUS AGENTS AND THEIR RISK GROUPS
Avian Influenza
RISK GROUP 2/3 - Virus
___% Equipment failure
___% Human related factors
- 20% Equipment failure
- 80% Human related factors
Hazards Associated with Recipient/Host, give two…
● Consequences of exposure
● Host susceptibility
● Immune status
● Modification of host range
● Pathogenicity of host strain
INFECTIOUS AGENTS AND THEIR RISK GROUPS
Salmonellosis
RISK GROUP 2 - bacteria
