Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MBG 2040 > Lectures 16-18 - Gene And Chromosome Structure > Flashcards

Lectures 16-18 - Gene And Chromosome Structure Flashcards

1
Q

What are nucleic acids composed of?

A

Nucleotides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

DNA is composed of

A

Nitrogen containing bases (A,T,G,C) and U, sugars, phosphate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Which are purine?

A

A and G

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Which are pyryimidine?

A

C, T, U

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

How are nucleotides joined?

A

Phosphodiester bond

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What does the phosphodiester bond link?

A

C3 and C5 to another sugar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How many h bonds between T and A

A

2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

How many H bonds between C and G?

A

3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Two strands of dna and polarity?

A

Opposite polarity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Techniques for dna and rna analysis?

A

Molecular hybridization
Gel electrophoresis
Restriction enzymes and cloning
PCR
Next generation sequencing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

PCR?

A

Forward primer: Anneals to the bottom strand and has the same sequence as the top strand
Reverse primer: anneals to the top strand and has the same sequence as the bottom strand

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is super coiling?

A

DNA double helix curls again

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How does dna become super coiled?

A

Single strand nick in dna allows it to form figure 8 and supercoil

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Genomes comparison?

A

Eukaryotes have large genomes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is chromatin?

A

DNA+histones+protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What are the three levels of dna packaging?

A

Nucleosomes
30 nm chromatin fibre
Inter-phase chromosomes

17
Q

First level of dna packaging?

A

DNA wraps around nucleosomes, 1 3/4 times and are linked by linker dna, it is 11nm in width

18
Q

What are nucleosomes consisting of?

A

8 histones

19
Q

What is the second level of packaging?

A

Nucleosomes with linker dna folds into 30 nm diameter chromatin fibre

20
Q

What is the third level of packaging?

A

30 nm fibre attaches to chromosome scaffold which folds and twists, it is 300 nm in diameter

MBG 2040 (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions