lecture quiz 6

Question 1

You are interesting in following the expression of Splice Variant 2 (bottom) of human SMNDC1. In the graphic view on NCBI, exons and introns are numbered in the order they are transcribed. Using quantitative PCR, what region of splice variant 2 would you use to design your primers to only detect splice variant 2.

Answer 1

exon 2

Question 2

You would like to amplify the portion of your gene that corresponds to ONLY the mature mRNA using the polymerase chain reaction.

Which template should be used?

Answer 2

complementary DNA

Question 3

You would like to amplify the entire exon that contains the ATG that corresponds to the start of translation for your gene, using the polymerase chain reaction.

Which template could be used?

Answer 3

genomic DNA or complementary DNA

Question 4

You would like to amplify the promoter for your gene using the polymerase chain reaction.

Which template could be used?

Answer 4

Genomic DNA

Question 5

You would like to amplify the entire unique portion of the coding region of the BOTTOM splice variant (splice variant 2) for the human cytochrome b-245 alpha chain (CYBA) using the polymerase chain reaction. Which primers would you use.

Answer 5

AGCCAAGT
CTAGCTGT

Question 6

Which of the following technologies in your Toolbox use enzymes isolated from either bacteria, viruses or eukaryotic cells?

• designing primers to amplify your gene of interest
• ligating your gene into a plasmid
• separation of DNA by gel electrophoresis
• cutting of DNA
• polymerase chain reaction
• sequencing DNA
• creation of probes for microarray analysis

Answer 6

• ligating your gene into a plasmid
• cutting of DNA
• polymerase chain reaction
• sequencing DNA
• creation of probes for microarray analysis