Lecture 9: Sources and Genetics of Crop Quality Traits     Flashcards

1
Q

Importance/ utility of crops and plants

A

Oxygen, cleansing air

Fuel and materials, industrial

Soil conservation

Food and nutrition

Biodiversity and ecosystem stability

Beauty, Health and wellbeing

Pharmaceuticals and drugs

Climate regulation and Carbon sequestration

Habitats, sanctuaries, supporting
wildlife / pollinators

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2
Q

Careers in plant biology

A

Research and development
Breeder
Entrepreneur
Scientific communication/ policy advisor
Molecular engineer/ biotechnologist
Teacher/Lecturer
Patent agent/Attorney

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3
Q

Lecture content

A

Quality traits in crops

Genetics of traits

Priorities for improving nutritional content

Breeding and transgenic approaches: Biofortification and the Golden rice project

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4
Q

Definition of ‘crops’

A

‘Plants that can be grown and harvested extensively for profit or subsistence.’

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5
Q

Definition of agronomy

A

Crop science; plant breeding, physiology, biochemistry, soil science, etc

Science and Economics of Crop Production

‘agros’=field;‘nomas’=manage

Synthesis of principles: Environmental science; meteorology, ecology Farm and field management, Economics

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6
Q

Johan Kroon’s Background is in metabolic study:

A

The chemical and physical processes
that allow organisms to build up and
break down molecules to grow,
develop, survive and reproduce.
· Synthesizing substances to create building
blocks and energy.
· Transforming substances into energy.
. Making energy available for use.

+he has worked in the engineering of metabolic pathways of oils and lipids
+bioderived chemicals and polymers

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7
Q

Classification of crops or plants

A

Provides valuable knowledge about optimal conditions necessary for successful cultivation:

Specific requirements; appropriate soil & water conditions, adaptability, growing habits, and climatic needs

Understand economical production & usage of crop plants and growing seasons

Classification can be based on:

Climate

The Growing Season

Ontogeny (‘life cycle / duration’)

Agronomic Classification of Crops (use and characteristics)

Photosynthesis

Reduction of Carbon Dioxide /Dark Reaction)

Cultural Method/Water

The Number of Cotyledons

Root System

Economic Importance

The Length of Photoperiod Required For Floral Initiation

Some plants will have multiple possible classifications

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8
Q

Classification based on climate

A

Tropical climate crops - warm and hot climate e.g. rice, and sugarcane

Temperate crops - cool climate e.g. wheat, oats, gram, and potatoes

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9
Q

Classification based on the growing season

A

Rainy crops. warm and wet weather during their growth period and short day lengths for flowering.
e.g. cotton and rice

Cold-season crops. winter season / cold and dry weather and longer day lengths for flowering.
e.g. wheat, gram, and sunflower

Summer crops. warm weather during the major growth periods and longer day lengths for flowering.
e.g. Groundnuts, watermelons, pumpkins, and gourds

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10
Q

Classification Based on Ontogeny
(‘life cycle /duration’)

A

Seasonal - complete their life cycle within a single season, typically summer. E.g. rice and wheat

Two-seasonal e.g. cotton, turmeric, and ginger

Annual require a full year to complete their life cycle. E.g. sugarcane

Biennial grow in one year and flower, fructify, and perish the next year.
e.g. radish, carrot, beet or cotton, turmeric, and ginger, bananas, papaya

Perennial live for more than one year. occupying land more than 30 months.
e.g. ginger, garlic, sweet potatoes, fruit crops such as mango, guava

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11
Q

Agronomic classification of crops
(by use and characteristics)

A

> Grain crops: refer to cultivated grasses grown for their edible starchy grains, cereals like millet

> Pulse/legume crops: are leguminous crops whose seeds are used as food. On splitting, they produce crops rich in protein, green gram, black gram, soybean, pea, and cowpeas

> Oil seed crops: are crops whose seeds are rich in fatty acids and are used to extract vegetable oil to meet various requirements. groundnut, mustard, sunflower, sesamum, and linseed

> Forage crops: refer to vegetative matter, fresh or preserved, utilized as food for animals. Crops are cultivated and used for fodder, hay, and silage, sorghum, elephant grass, guinea grass, and other pulses.

> Fiber crops: are grown for their fiber yield, which may be obtained from seeds, such as cotton

> Root crops: are crops whose economic production is in their roots, sweet potatoes and carrots

> Tuber crops: are crops whose edible portion is not a root but a short thickened underground stem, potatoes and yams

> Sugar crops: refer to the two important crops which are cultivated for the production of sugar, sugarcane and sugar beet

> Starch crops: are grown for the production of starch, tapioca and sweet potato

> Dreg crops: are used for the preparation of medicines, tobacco, mint, and pyrethrum

> Spices and condiments/spices crops: refer to crops whose products are used to flavor, taste, and sometimes color fresh preserved food. ginger, garlic, chili, cumin, onion, coriander, cardamom, pepper, and turmeric

> Vegetable crops: may refer to leafy or fruity vegetables, such as tomatoes

> Green manure crops: are grown and incorporated into the soil to increase soil fertility.

> Medicinal and aromatic crops: refer to medicinal plants, such as cinchona, and aromatic plants, such as lemon grass, citronella grass, cannabis, mint, peppermint, rose, jasmine, and henna

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12
Q

Classification Based on Cultural Method/Water

A

e.g. Rainwater, Irrigated. Chili, sugarcane, Banana, and papaya

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13
Q

Classification Based on Root System

A

A taproot system; a main root that grows deep into the soil. E.g. grapes and cotton

Adventitious or fiber-rooted; shallow, fibrous roots that spread into the soil.

E.g. cereal crops, such as wheat and rice

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14
Q

Classification based on The Length of Photoperiod Required For Floral Initiation

A

Length of day and night - floral initiation = photoperiodism.

Three categories:

Short-day plants initiate flowering when days are shorter than ten hours
e.g rice, green gram, and black gram

Long-day plants require longer days, more than ten hours, for floral ignition
e.g. wheat and barley

Day-neutral plants are not influenced by photoperiod and their rate of flowering initiation depends on the duration of the photoperiod e.g. cotton and sunflower

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15
Q

Classification Based on Photosynthesis (Reduction of Carbon Dioxide /Dark Reaction)

A

(Classification: C3, C4 & Cam plants)

C3 Plants: use the C3 cycle to fix carbon from carbon dioxide into 3 carbon sugars.

Enzyme involved in primary carboxylation is ribulose-1,-biophosphate carboxylase.

Photorespiration is high in C3 plants, which results in lower water use efficiency

e.g. rice, soybeans, wheat, oats, rye, or hardgrass, barley, cotton, and potatoes

C4 Plants: fix carbon by producing four-carbon compounds such as malic acid or acerbic acid.

Phosphoenol pyruvic acid carboxylase - carboxylation and has a high affinity for carbon dioxide

= negligible photorespiration.

Higher photosynthetic rates than C3 plants for same amount of stomatal opening

= drought-resistant

e.g. Corn, sorghum, sugarcane, millet, and switchgrass

Cam Plants: Crassulacean acid metabolism; periodic water supply

open their stomata at night (air temperature lower) to fix a large amount of carbon dioxide as malic acid which is stored in vacuoles.
e.g. sedum, kalanchoe, pineapple, opuntia, and snake plants

In the day, stomata are closed, and carbon dioxide stored as malic acid is broken down and released

Negligible transpiration and are highly drought-resistant e.g. pineapple, sisal, and agave

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16
Q

Classification Based on The Number of Cotyledons:

A

Monocots or monocotyledons are crops with one cotyledon in the seed
= all cereals and millet (grass type)

Dicots or dicotyledonous are crops having two cotyledons in the seed
= all legumes and pulses

17
Q

Classification Based on Economic Importance

A

Cash crops are grown for the purpose of earning money e.g. sugarcane, cotton, cannabis

Food crops are grown to produce food grain for the population and fodder for cattle.
e.g. wheat and rice

18
Q

Quality traits

A

‘A property or aspect with association to one or more key performance characteristics in target environments’

Each crop or ‘cultivar’ has its own specific set of quality traits *.

e.g. yield, lodging (risk of falling over), disease or pest resistance, grain retention, thresh-ability, photoperiod sensitivity, end use quality, nutritional value

Climactic and environmental adaptations

Repeatability, genetic variation

19
Q

Concept of quality

A

Crop dependent – based on what we need to do with the crop:

*Varies from crop consumption to food consumption
*Market – nutritional and or industrial qualities
*Degree of Excellence/Suitability/Fitness of plant product for end use

Aspect of growth at any stage:

  • Nutritional worth
    *Market quality
    *Seeds
    *Processing
    *Transport & Storage
20
Q

Classification of quality traits

A

Morphological - shape and appearance

Organoleptic - sensory

Nutritional – food, feed and health

Biological – technofunctionality

21
Q

Morphological traits
& organoleptic traits

A

Morphological:
Easily observable – appearance related
Size and colour
Consumer acceptance e.g. ‘wonky’ produce fetches lower market value
Defines the market value of products

Organoleptic:
‘Being that which stimulates the senses’
Sensory qualities: Easily determined
Palatability of the produce: taste, aroma, smell, juiciness, texture, etc
Influences consumer acceptance

22
Q

Nutritional traits

A

Determine the value of the produce in human / animal nutrition
Paramount in human/animal health: but not easily appreciated/observed

Nutritional factors
Protein content & quality
Oil content & quality
Vitamin & mineral content

Also important: Presence of anti-nutritional factors e.g. in pulses

23
Q

Biological traits – technofunctionality

A

Technical and specific per crop – determines utility of crop for consumption

^ Not always directly obvious to producer or consumer

Examples:
*cooking quality of rice
*fibre strength of cotton
*Pathogen resistance
*Keep/storage/transportability of fruits and vegetables
*Protein efficiency ratio (PER) - biological value and body weight gain

24
Q

Genetic variability in plant breeding

A

Variation in the genetic makeup of individuals within a population:

different individual genetic traits present in a group of plants mutations, genetic recombination during sexual reproduction, etc

Raw material for selection and foundation for plant breeding programs:

*Adaptation changing environments
*Disease/Pest resistance
*Yield improvement
*Quality traits

Sources of Genetic Variability:

*Transgene / Biotechnology
*Wild species and relatives (of cultivars)
*Somaclonal variants
*Spontaneous / Induced Mutations
*Germplasm - Instrumental in Agri biodiversity Total of ALL the genes crop & related species
*Cultivated crop varieties - Most preferred! Maintained living genetic resource

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Genetic diversity in plant breeding
Broad perspective: Overall genetic composition/ total number different alleles of whole population Both richness of genetic traits within a population and the distribution of these traits Diversity Components: *Allelic (per gene locus) *Genotypic (combination alleles) *Population (trait variation among different populations of same species)
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Cultivated crop varieties
Crop and ornamental cultivars Cultivar = bred by humans to express distinct traits Variety = Natural selection Cultivar and variety examples: Brocolli – 164 types in North America Strawberry >600 types stemming from 5 or 6 wild species (rose) Malus domestica 'Apple' >7500 cultivars and >30,000 varieties Triticum aestivum 'wheat'>450 cultivars and >30,000 varieties
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Germplasm
Collection of LIVE plant matter, including seeds and spores - For conservation and use of biodiversity In situ conservation * Genetic resources conserved in natural habitat * Species maintained in original place e.g. in nature reserve Ex situ conservation * Conservation genetic resources outside natural habitat e.g. gene bank or botanic garden Germplasm steps:
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The Svalbard Seed Vault
🏔️ Svalbard houses Earth's seed bank, hidden deep in a mountain, 300 kilometers north of the Arctic Circle. 🌱 Over 1 million dormant seed samples from global crops and trees are stored in secure, cold vaults at -18 degrees Celsius. 🌍 The seed bank acts as a crucial reserve for biodiversity restoration in case of global emergencies. 🌨️ Svalbard's natural permafrost initially safeguarded the seeds, upgraded after a 2017 incident caused by melting permafrost. 🌐 Despite global seed deposits, Svalbard offers a unique, globally accessible backup for catastrophic scenarios. 🚢 Expeditions to Svalbard focus on rare wildlife and natural wonders, while locals share stories to raise awareness about planetary welfare. see: https://www.youtube.com/watch?v=k7NwXMo6wr8 & https://discoverwithus.hurtigruten.com/
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Steps for conservation
1. Exploration and collection of plant genetic resources 2.Evaluation and characterization of plant genetic resources 3.Conservation of plant genetic resources 3a Ex-situ conservation: - Seed storage, Gene bank storage, Botanical garden - In vitro conservation: DNA storage or Pollen storage 3b In situ conservation: - On farm, Field farm, Gene sanctuary, Biodiversity hotspots, Natural parks 4. Utilization of plant genetic resources in crop improvement programmes using different plant breeding and biotechnological approaches 5.Characterization and evaluation of plant genetic resources 6.Development/ Improvement crop varieties 7. And/ or Release of new crop varieties