Lecture 9 - Processing Flashcards

1
Q

BEFORE SPECIMEN PROCESSING - pre ana

A

Blood Collection
Positive patient ID
Labelling at the bedside
Getting the specimen to the lab

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2
Q

Barcoded Wristband

A

barcoded wristband when scanned, provides the patient’s information and ID number

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3
Q

Proper patient ID- Accreditation Canada Standards

A

Failure to properly identify patients can create adverse events such as blood transfusion errors, diagnostic testing errors, wrong person procedures, etc.
Accreditation Canada Standards : two client identifiers to ensure positive patient identification (PPID) and patient safety.

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4
Q

Two standards

A

The first is the verbal confirmation that you have the right person using two identifiers
Unique ID number+ full name or
full name + unique ID /DO

The second component is to use the same two identifiers to confirm that the service or procedure was intended for the specific patient –
checking ID against a source like the wristband, requisitions, and labels.

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5
Q

Barcode scanning

A

eliminates human errors in patient identification, it speed up treatments, lower costs, and guarantee patient safety.
Know what is on a barcode label very important

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6
Q

Specimen Transport Bags

A
Standard Precautions
Specimen transported to the lab in plastic  leak proof bags 
Biohazard signs
Never place requisition 
inside bag with specimen.
Place in the outer pouch
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7
Q

Unacceptable

A

Multiple patients with more than one type of requisition per bag.
Spilled sample
Requisition inside the bag

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8
Q

Specimen Receiving/ and Send out

A

Once the specimen is collected it is sent to the lab for testing
Specimen arrival time is tracked by the LIS
Technicians receiving the specimen is tracked by the LIS
Technicians doing the phlebotomy is tracked by LIS
After testing  specimen storage, retrieval and send out is tracked

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9
Q

Logging and Reporting Process

A

Pre analytic function
Accurate logging and reporting process must be in place
User ID captured
LIS is interfaced with the analyzers

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10
Q

Functions of LIS

A
Preanalytic- Post analytic 
Proper ID of patient
Test ordering or order entry
Specimen labels
Specimen accessioning
Specimen receiving/ and tracking
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11
Q

Order Entry-Specimen Barcode or Manual Entry

A
Patient information
Name of doctor, receiving the report
Test request time, date
Tests to be performed
Collection time and collectors initial
Name of person entering the test
Test priority
Any specimen comment
NB * a paper requisition or test request form requires the same information as an electronic order
The LIS will generate a work list with the collection information
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12
Q

Specimen Accessing- ID number

A

Number generated by the LIS for each patient specimen
Numbers represented in the bar coding
Process can be generated manually

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13
Q

Aerosol Prevention

A

To prevent aerosol when a stopper (cap) of a blood collection container is opened, cover top of disposable gauze
Hold tube away from the body
Tube caps are removed behind the shield or biosafety cabinet
Shields acts as a barrier and prevents aerosol entering eyes, nose and mouth.

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14
Q

Biosafety Cabinet

A

Sash no more than 12”

Keep clear so max air flow

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15
Q

centrifugation

A

Tubes should be kept capped
Centrifuge cover must be kept in place and left on until the centrifuge stops on its own
Keep on level counter

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16
Q

Handling Sharps and Pipettes

A

Needles in sharp containers only
Disposable pipettes and pipette tips must be placed in disinfectant in sharp container
Autoclave or dispose according to the laboratory’s protocol (SOP)

17
Q

Specimen Handling and Shipping Requirements

A

Proper handling and PPE is vital according to Standard Precautions Policy)
Safety of all who come into contact with the specimen must be guaranteed
Shipping container must meet Federal and Provincial regulations (TDG)
Amber bags used for light sensitive specimen
Bags must have biohazard symbol and pocket for requisition
Requisition placed in the outside pocket of the bag ONLY
Proper transport containers according to provincial and federal regulations

18
Q

Preservation of Specimen

A

Maintaining specimen at correct pre-analytic temp is extremely important
Some products have re-usable cold pack gel for specimen requiring cool temperatures
Errors in specimen collection and handling (pre-analytic)
errors are a significant cause of incorrect patient results

19
Q

Preservation and Storage of Specimens

A

Fresh specimen is ideal
Some specimen must be preserved until testing is done

Temperature ( 4 ̊C) fridge
Lower temperatures, greater stability of chemical constituents
Growth of bacteria inhibited
Specimens must be brought to RT before analysis

Freezer (-5 °C)
Serum or Plasma
DO NOT freeze Whole blood - RBC’s are ruptured and lysed
Specimen must be brought to RT before analysis