Lecture 9

Question 1

Culture of animal cells require what to grow

Answer 1

nutrientrich media and special solid surfaces (may be pre-coated with extracellular-matrix, ECM, proteins)

Question 2

What are primary cells

Answer 2

cells isolated directly from

tissues.

Question 3

What are commonly used to establish primary cell culture

Answer 3

Normal animal tissues (e.g., skin, kidney, liver) or whole embryos

Question 4

What are used to break cell-cell and cell-matrix interactions

Answer 4

Proteases + EDTA (Ca2+ chelator, inactivates cell-adhesion molecules, CAMs)

Question 5

After breaking cell-cell and cell-matrix interactions, the released cells are then placed in

Answer 5

nutrient-rich, serum-supplemented medium in dishes, where they can adhere to surfaces and to one another