Lecture 8 - Photosynthesis Flashcards

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1
Q

What are autotrophs?

A
  • use inorganic carbon as a carbon source to fix carbon into organic compounds
  • photoautotroph = uses light energy to fix carbon
  • chemoautotroph = uses redox reactions to fix carbon
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2
Q

What are heterotrophs?

A
  • use organic carbon as a carbon and energy course
  • chemoheterotroph = use organic carbon as the main nutrient
  • photoheterotroph = uses light energy and organic carbon (RARE)
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3
Q

Where does photosynthesis occur?

A
  • the chloroplast
  • arose from endosymbiosis theory
  • double membrane-bound with stacks of internal membrane called thylakoids
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4
Q

What is the reaction of photosynthesis?

A

6 carbon dioxide + 6 water –> glucose + 6 oxygen

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5
Q

Where do light reactions and light-independent reactions occur?

A
light = thylakoids 
light-independent = stroma and carbon fixation
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6
Q

What is the point of light reactions?

A

use energy carried by light and convert it into chemical energy in the form of ATP and NADPH that will be used to power the Calvin cycle

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7
Q

What happens to light that is absorbed?

A
  • released as heat or fluorescence
  • absorbed light can be captured and used as an energy source
  • photosystems capture light energy and convert it into chemical energy
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8
Q

What are photosystems?

A
  • composed of a protein complex called the reaction center
  • surrounded by several light-harvesting complexes
  • sit in the thylakoid membrane and contain pigments
  • pigments within the photosystem can absorb light, excite electrons, and pass those electrons through the photosystem
  • convert light energy into chemical energy in the form of an excited electron
  • review diagram**
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9
Q

What is the ETC?

A
  • electron transport chain
  • a set of increasing strength of electron acceptors
  • allows energy from excited electrons to be converted into a proton gradient and released in small discrete steps
  • exists between photosystem 2 and 1
  • excited electron is passed down the ETC and releases energy from the redox reactions that are used to pump H+ into the thylakoid lumen
  • excited electron from photosystem one is the used to reduce NADP+
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10
Q

What is chemiosmosis?

A
  • formation of ATP in photosynthesis using ATP synthase powered by a [H+] gradient
  • in photosynthesis = photophosphorylation
  • in cellular respiration = oxidative phosphorylation
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11
Q

What is ATP synthase?

A
  • an enzyme complex that couples an endergonic reaction with an exergonic process
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12
Q

What is NADPH?

A
  • an electron carrier, sources of energy because they can reduce molecules
  • carries electrons between redox reactions (from light reactions to Calvin cycle)
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13
Q

What is the Calvin Cycle or Dark Reactions?

A
  • uses energy from light reactions (ATP and NADPH) to convert carbon dioxide to sugar
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14
Q

What is the point of dark reactions?

A
  • fix carbon
  • “fixation” is the conversion of an atmospheric nutrient to organic form = highly endergonic
  • anabolic
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15
Q

What are the three phases of the Calvin Cycle?

A

1) Carbon Fixation
2) Reduction
3) Regeneration

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16
Q

What happens during Carbon Fixation?

A
  • one carbon dioxide molecule is added per turn of the cycle
  • turn three times to make one molecule of G3P
  • enzyme = Rubisco = fixes carbon
17
Q

What happens during reduction?

A
  • there are two 3 carbon products of Calvin Cycle
  • G3P = glyceraldehyde - 3 - phosphate
  • two G3P = one glucose
  • reduced by electrons from NADPH
18
Q

What happens during regeneration?

A
  • RuBP (ribulose (5C) biphosphate) is regenerated
  • RuBP is the substrate for Rubisco
  • a set of complex reactions requiring ATP
19
Q

What are the net inputs to the Calvin Cycle?

A
  • 3 carbon dioxide
  • 6 NADPH
  • 9 ATP
20
Q

Know the reaction of Rubisco and the overall reaction of Calvin Cycle

A
  • refer to notes
21
Q

Do we require more ATP than NADPH?

A

yes, we need more ATP to drive the Calvin Cycle BUT light reactions make ATP and NADPH in equal qualities

22
Q

What is the point of Cyclic Electron Flow?

A
  • to make ATP without making NADPH to balance the needs of Calvin Cycle with supply from light reactions
  • regulated by [ADP], if it increases, then cyclic electron flow occurs
  • increase in ADP means a decrease in ATP