lecture 8 ( Intracellular Compartments and Protein Transport) Flashcards
Protein are transported to four targets, which is:
1) stays in the cytosol (no targetting sequence)
2) goes to the ER (targetting sequence to the ER)
3) goes to the nucleus (nuclear pores)
4) goes to the mitochondria ( outermembrane/ innermembrane/ matrix)
Amphipathic N-terminal signal sequence is:
rich in hydrophobic and hydrophilic amino acid located in the matrix targeting sequence.
what is the name of the general import pore in the outermembrane?
TOM
what is the name of the general import pore in the innermembrane?
TIM
which region does the protein import occurs in mitochondria (to the matrix)?
the region where the outermembrane is in close contact with the innermembrane
what are the 3 translocators associated with the transfer of protein to the matrix (innermembrane) ?
Tim 23/17 & Tim 44
what is the name of the translocator (import pore) that imports the protein to the intermemebrane from the cytosol?
Tom 40
how is the targeting sequence removed after reaching the matrix?
by matrix protease
how does the Hsc 70 help drive the import into the matrix?
the ATP hydrolysis act as a heat shock which pulls the protein inward
True or False: Folding of some proteins doesn’t depend on the matrix chaperonins
False, the matrix chaperonins interacts and protect the unfolded form of protein.
True or False: Protein imported to the peroxisome from the ER transports using vesicles.
TRUE
In the peroxisome the oxidation of substrate forms:
Hydrogen Peroxide (H2O2)
What enzyme degrade H2O2 to H2O in ther peroxisome?
Catalase Enzyme
True or False: H2O2 can damge the plasma membrane
False, it damges the DNA
True or False: the protein destined to the peroxisome will be unfolded and the targeting sequnce will be removed.
False, the protein will be in it’s folded form and the sequnce will not be removed
