Lecture 7 Flashcards
Nausea vs. vomiting - which is the ‘clinical sign’?
Vomiting
What are the four broad methods of AGENT identification?
Visualisation e.g microscopy
Nucleic acid/genome detection e.g PCR
Haemagglutination (if it causes that)
Protein/antigen detection e.g immunohistochemistry
Is ‘deep sequencing’ available commercially yet?
No
Name 5 different methods for ANTIGEN detection. Which of these require VIABLE organism?
ELISA Agglutination assay Immunofluorescence* Immunohistochemistry* Immuno-electron microscopy*
- because need to do cell culture first
(check this..)
You take acute serum on day 3 since the start of clinical signs. When should you take the convalescent sample?
Day 13 - 17
10 - 14 days later
What is ‘virus neutralisation’ detecting? Antigen or antibody?
Antibody (in sample)
What is the purpose of the ‘virus concentration controls’ in virus neutralisation assay?
Ensure you havent added so much virus you are obscuring antibody effect, or so little you are not seeing CPE anyway.
What do the two sera in the virus neutralisation assay represent?
Acute and convalescent sera
What does the ‘cell control’ do in a virus neutralisation assay?
Ensures cell culture is healthy.
What do the ‘test serum controls’ in a virus neutralisation assay show you?
The sera isn’t capable of damaging the cell monolayer alone.
What does the haemagglutination inhibition assay detect?
Antibodies - inhibit agglutination activity of a known virus
If you are making twofold serial dilutions for a HAI assay, and your last well without agglutination is well number 4, then that is the titre of your antibody in the sample?
80 HIU
What kind of ELISA detects ANTIGEN in a sample?
A capture/sandwich ELISA
What kind of ELISA detects ANTIBODY in the sample?
An indirect ELISA
What is the technology behind Snap ELISA tests called?
Lateral Flow Immunochromatography
