Lecture 6: The Proteome Flashcards
1
Q
What are the common physical interactions involved in protein folding ?
A
Breakdown causing aggregation
2
Q
What are the common physical interactions involved in secondary structure formation ?
A
Hydrogen bonds
3
Q
What is the proteome ?
A
The protein complement encoded by the genome
4
Q
What is proteomics ?
A
Any protein based approach that has the capacity to provide new information about proteins on a genome wide scale
5
Q
What are proteomics main objectives ?
A
- Identifying and quantifying proteins
- Determing how proteins interact and form networks
- Outline the precise 3d structure of proteins
- Provide functional annotations for the entire proteome
6
Q
How might you identify and quantify proteins ?
A
- Gel based fractionation
- Mass spectrometry
7
Q
What are the three modules of mass spectrometry ?
A
- ion source
- mass analyser
- detector
8
Q
How would you determine protein protein interactions ?
A
- Two hybrid screening
- Protein affinity chromatography
9
Q
What are the three main methodologies of structural biology ?
A
- x-ray cyrstallography
- NMR specroscopy
- Electron microscopy
