Lecture 6 Reading

Question 0

DNA polymerase

Answer 0

The first nucleotide polymerizing enzyme

Question 1

DNA templating

Answer 1

Mechanism cell uses to copy nucleotide sequence of one DNA strand into a complementary DNA sequence.

Question 2

3’-to-5’ proofreading exonuclease

Answer 2

Clips odd any unpaired or mispaired residues at the primer terminus, continuing until enough nucleotides have been removed to regenerate a correctly base paired 3’-OH terminus that can prime DNA synthesis

Question 3

DNA primase

Answer 3

Uses ribonucleoside triphosphate to synthesize short RNA primers on the lagging strand

Question 4

What two replication proteins are needed to open double helix

Answer 4

DNA helicase and single-strand DNA binding proteins

Question 5

Single strand DNA bind proteins

Answer 5

Stabilize unwound conformation of DNA

Question 6

Sliding clamp

Answer 6

Keeps polymerase firmly on the DNA when it is moving, but releases it as soon as the polymerase runs into a double strand region of the DNA

Question 7

Clamp loader

Answer 7

Special protein complex that Hydrolyzes ATP as loads the clamp on to a primer-template junction

Question 8

Initiator proteins

Answer 8

Begin process of DNA replication by binding to double strand DNA and prying the two strands apart, breaking h bonds bet, bases

Question 9

Replication origins

Answer 9

Position at which dna helix is first opened