Lecture 6 Basic techiques Flashcards
1
Q
What is CRISPR/Cas9?
A
Adaptive immune systeem in bacteria/archaea against viruses
2
Q
How was crispr discovered?
A
Sequencing of the E.coli genome revealed:•Short repeating sequences•Interspaced with unique sequences: spacersAccompagnied by a number of Cas genes => Cas proteinsCas genes encode DNA cutting enzymesSpacer sequences showed similarity to viral sequencesMay be a defense mechanism?
