Lecture 6-9 Flashcards
What is biological evolution?
Gradual change in the inherited traits of a population.
Survival: maintain internal homeostasis, respond to external stimuli, consume and produce energy
Reproduction: reproduce and have a form of heredity
Survival + reproduction = natural selection
Change in allele frequencies in a pop. over time
Driven by variation in reproductive success (fitness)
What is the Darwinian Revolution?
Publiction of “On the Origin of Species”
1) The tree of life: all species on Earth have evolved from other species (perhaps, ultimately, from just one)
2) Natural Selection: organisms are well-adapted to their environments because they accumulate, over the generations, traits that enable them to survive and/or reproduce better than organisms lacking those traits
What is natural selection?
Individuals in a pop. differ in their traits
Some traits confer an advantage (in a given environment)
- by that advantage, those traits allow certain individuals to survive and reproduce more
Individuals that have these traits survive and reproduce better than others
- i.e. selection is on phenotype (expression of trait)
If differences are heritable, the frequency of advantageous traits will increase in the next generation
- i.e. evolution is due to changing “genotype” (genetic coding for traits)
Gene definition
A self replicating DNA unit that occupies a specific location on a chromosome and determines a particular characteristic in an organism
Allele definition
A variant (different) form of a given gene (section of DNA) that codes for smt (e.g. a trait)
What is fitness in terms of biological evolution?
Fitness translates to reproductive success
I.e. how many surviving offspring does one have compared to others in the pop.
What is a phenotype?
Interaction between genotype and environment
Even individuals with the same genotype have different expressions depending on the environment (plasticity)
Why do individuals’ genetics vary?
mutuation
mode of reproduction
How do prokaryotes transfer info from generation to generation?
An enzyme gently break apart fhe two DNA strands
Other enzymes attach complementary bases to each of the old strands
Another enzyme checks for mistakes (proof-reading) and a DNA repair enzyme fixes them
Result: two strands virtually identical to the original
Mutation: mistakes happen! inital source of all variation
What is the reproduction in prokaryotes?
Binary fission
Replication of the circular chromosome followed by fissioning of the cell
Transmission of DNA-coded info across generations
DNA replication: cell fission (splitting) placing replicated DNA into daughter cells
What is binary fission?
Asexual reproduction in prokaryotes, creates new prokaryotes and some genetic diversity via mutation (identical apart from mutations)
What are some other ways prokaryotes transmit genetic info (not equal to reproduction)?
All do not = reproduction, but introduces genetic variation
Conjugation: sharing plasmids (separate ring of DNA)
Transformation: a prokaryote picks up a plasmid (genetic material) from the environment
Transduction: a virus relocates DNA from one prokaryote to another via viral replication cycles
What is different about the genetic structures of eukaryote cells?
Genetic material organized into multiple linear chromosomes
Each chromosomes consists of one long molecule of DNA
After DNA replication, two identical “sister chromatids” form
What is mitosis?
Duplicate chromosomes lines up and are pulled to opposite sides of parent cell
The cell then divides (fission) to produce daughter cells
What are the steps of asexual reproduction in a single-celled eukaryote?
Start off with a haploid (N) cells - 3 chromosomes
Duplication: chromosomes and DNA duplicate
Mitosis/fission: results in two identical haploid (N) cells with 3 chromosomes
What are the steps of sexual reproduction?
Gametes: 2 haploid (N) cells, each with 3 chromosomes but from different mating strains (2 parents)
Gametes fuse to form diploid (2N) zygote
6 chromosomes become uncondensed inside the new nuclear membrane
Meiosis: we start off with a diploid (2N) zygote
Reassortment or reassortment + recombination: Chromosomes duplicate, and homologous chromosomes line up in center of cell (random process )
Reassortment: homologous chromosomes separate and move to opposite sides of cell
2 groups of 3 chromosomes (each with replicated sister chromatids)
Sister chromatids separate
Now 4 groups of 3 chromosomes each
What creates more possible combinations in gametes during reassortment?
More chromosomes
Example:
N=2 –> 4 possible gametes
N=3 –> 8 possible gametes
N=4 –> 16 possible gametes
What are the benefits of sexual reproduction compared to asexual reproduction?
Generates a lot of variation
What are the consequences of sexual reproduction?
Mate searching costs
- as pop. size grows smaller, probability to find a mate decreases –> can lead to extinctions
Competition
Display costs
Only half of pop. generates offspring
What conditions favour sexual reproduction?
High number of environmental factors (complex)
high genes per trait
Low background mortality
Strong soft selection
Low mutation rate
Periodic catastrophes
What is hard selection?
Extra mortality (reduced reproduction) for maladapted individuals
What is soft selection?
No extra mortality, but who dies depends on fitness of other individuals
What are direct advantages of sex?
DNA repair mechanism
Masking mutations – higher chance that one copy works
What is the biochemical evidence for Archean ancestor to eukaryotes?
DNA sequence data from genes in the nucleus of eukaryotes suggests that eukaryotes are more closely related to Archeae than to bacteria
What is the biochemical evidence for bacterial ancestor to eukaryotes?
Mitochondria and chloroplasts (eukaryote organelles) have their own circular DNA and prokaryoteic-type ribosomes. Most similar to bacteria
What is endocytosis?
A substance gains entry into a eukaryotic cell; the cell membrane wraps itself around the particle and pinches off a vescicle inside the cell
Steps:
- Food particle taken in by endocytosis
- Fusion forms secondary lysosome
- Food particles digested
- Products of digestion
- Exocytosis of waste
Is simple observation and measurement sufficient to assess descent using comparisons involving phenotypic traits?
No, it does not account for environmental influence on phenotpes, ontogenetic changes, identification of “derived” traits. There is also insufficient data from living and fossil organisms to unambiguously trace changes in time as well as a lack of universal traits.
What needs to happen to have the chance of finding a fossil?
Chance of dying in one piece, being fossilized, remaining undisturbed, being exposed, being found, and being recognized.
The probability of all of these events occurring is extremely small.
What are the advantages of assessing descent through comparisons involving molecular traits?
Nucleotide sequences provide a direct record of all info stored in the genome.
No environmental or ontogenetic effects on the observed traits
Potential for universal traits
What are the disadvantages of assessing descent through comparisons involving molecular traits?
Traits observed only with use of sophisticated technology.
Inferring patterns of change in time not intuitive.
Back mutation at a site in the sequence is possible and complicates analysis.
Assuming constant molecular clock.
What are the steps to reconstructing phylogeny (lines of descent)?
1) Acquire nucleotide sequence data
- sampled part of a genome depends on how far back in time the relationships of interest lie
2) Align sequences from different organisms
- allow for mismatches due to point mutations, insertions, and deletions
3) Reconstruct most likely lines of descent
- assess the minimal number of steps requires to change from one sequence to another and use this as a measure of relatedness across all the organisms in the analysis
What are Acritarchs?
Early marine plankton.
A group of eukaryote fossils probably including algae, ciliates, dinoflagellates, radiolarians, foraminiferans.
Many acritarchs had tests (shells)
- example of structural complexity
- better source of fossil material
Why did diversity increase and what were the processes involved?
Increase in size of genome (more proteins, enzymes coded for)
Sexual reproduction (meiosis increases possible variation/mixing)
Increase in structural complexity (locomotion, protection)
Ecological changes (O2, energy sources, physical/biological landscape, photosynthesis in shallow water)
What are the origins of multicellular life?
Colonial protists and algae illustrate intermediate stage of complexity
Colonial life allows evolution of separate functions for individual cells
- feeding, reproduction, locomotion, etc.
- different metabolic pathways turned on and off
Ediacaran diversity
Variety of forms (vendian animals)
- leaflike fronds, round pads, worms
Some resemble jellyfish, sponges
Most are unique and unlike known animals
What was the ediacaran seascape in the late precambrian era (around 570 MA)?
Small shelly fossils in ocean sediments
Soft parts not preserved –> no idea what organism construced the shells
Burgess Shale Diversity
Ancestors of many modern groups
Arthropods, worms, sponges, jellyfish
But also many mystery organisms (failed lines of evolution? victims of chance?)
What is the Pikaia?
Ancestor of chordates, vertebrates, mammals and humans
What happened during the Cambrian Explosion?
Diversification in the animal kingdom
From Ediacarans (+/-570 MA) to early Cambrian animals (+/-540 MA)
All modern animal body plans established in less than 25 million years
Changes since then are just variations on those established plans
What is fitness landscape?
used to visualize the relationship between genotypes and reproductive success
What defined the Cambrian ecology?
Burrowing priapulid worms
Predators of small molluscs with spines
Swallowing prey all face the same way
Well-developed feeding behaviour
Give an example of a predator-prey “arms race”
Predator behaviour –> burrowing
Dig for prey –> hard sclerites
Jaws –> spines/completes shell
Modified hunting behaviour
Is there purpose in evolution?
No
Variations arise through chance mutations
Some mutations are positive, some negative, many neutral
Selection, not the organisms, decides which variations will survive of succees
Always interpret evolution in terms of chance variations, selection, probabilities of survival, not purpose and progress towards an objective
What does the predator-prey arms race mean in terms of evolution?
Interpret the sequence of events in the predator-pre arms race in the context of random mutations, variations and selection
Co-evolution
What can mutations create variations in?
Structure of the exoskeleton or skin, mouthparts, organs of locomotion, sensor structures
Behavioural traits
Physiological and metabolic pathways