lecture 6 Flashcards
1
Q
what is a catalyst
A
increase rate of reaction but no effect on equilibrium
2
Q
how does catalyst work
A
lower activation energy
stabilising transition state
rate increased
3
Q
three steps of enzyme bonding
A
substrate bonding
chemistry or catalysis
product release
4
Q
mechanism of chymotrypsin
A
enzyme creates nucleophile from serine side-chain
nucleophile attacks
covalent intermediate formed with second product bonded to serine and first product released
nucleophile made from water
attacks covalent intermediate breaking covalent bond to serine
second product released
5
Q
which dipeptides clipped out
A
14-15 and 147-148
6
Q
what amino acid active in chymotrypsin
A
serine 195
7
Q
steps of chymotrypsin mechanism
A
substrate binding creation of nucleophile nucleophile attack formation of acyl-enzyme creation and use of new nucleophile deacylation step
