lecture 5 Flashcards
what does CD stand for
circular dichroism
what is CD
optically active matter differentially absorbs left and right hand circular polarised light
what is CD used for
monitoring loss of secondary structure when denaturing
what characterises globular protein
variety of secondary structures
spherical shape
good water solubility
catalytic, regulatory or transport role
characteristics of fibrous proteins
one dominating kind of secondary structure
long narrow rod like shape
low water solubility
role in tissue/cellular structure
rules of a globular protein
hydrophobic groups in the middle
beta sheets are twisted into barrel structures
different ways polypeptide can turn corners
not all parts of the protein can be classified
what is the native state of a protein
the most thermodynamically stable state
what is anfinsen’s experiment
using enzyme to cut up RNA denature protein then renatures
RNA is enough
what is enthalpy change for denaturing
large and negative
entropy change in denaturing
large and negative
enthalpy change in a solvent
little change
entropy change in solvent
positive cause of hydrophobic effect
what is a folding funnel
finding the lowest energy fold by following it lower energy
