Lecture 5: Staining

Question 1

91. Define staining

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Question 2

92. State the purpose of staining

Answer 2

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Question 3

93. What is the most commonly used stain in histo? Why?

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Question 4

94. Give the 2 groups of dye classification

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Question 5

95. Describe the 3 types of dyes and the type of charge they carry

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Question 6

96. State the 4 methods of staining and give examples of each

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Question 7

97. Name the 3 techniques of staining and give examples of each

Answer 7

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Question 8

98. What is the difference between routine staining and special staining

Answer 8

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Question 9

99. What does vital staining demonstrate

Answer 9

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Question 10

100. List factors that affect staining

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Question 11

101. Define counterstain

Answer 11

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Question 12

102. Define – acidophil, basophil, argentaffin, argyrophyll, metachromatic and give example for each

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Question 13

103. Name the oxidiser in prep for haematoxylin

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Question 14

104. Define and name the mordant in prep for H+E

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Question 15

105. Give the 3 classifications of H+E and give3 examples

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Question 16

106. Define – regressive and progressive staining, differentiation + give examples

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Question 17

107. What is ‘blueing’?

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Question 18

108. Give example of blueing agent

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Question 19

109. Describe how to perform an H+E stain

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Question 20

110. State the end result of H+E

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Question 21

111. State precautions taken during reagent prep

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Question 22

112. Name the 3 types of Mountant used in histo lab

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Question 23

113. List the information displayed on a reagent label – state importance of these information

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