Lecture 5: Staining Flashcards
1
Q
- Define staining
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2
Q
- State the purpose of staining
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3
Q
- What is the most commonly used stain in histo? Why?
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4
Q
- Give the 2 groups of dye classification
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5
Q
- Describe the 3 types of dyes and the type of charge they carry
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6
Q
- State the 4 methods of staining and give examples of each
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7
Q
- Name the 3 techniques of staining and give examples of each
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8
Q
- What is the difference between routine staining and special staining
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9
Q
- What does vital staining demonstrate
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10
Q
- List factors that affect staining
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11
Q
- Define counterstain
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12
Q
- Define – acidophil, basophil, argentaffin, argyrophyll, metachromatic and give example for each
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13
Q
- Name the oxidiser in prep for haematoxylin
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14
Q
- Define and name the mordant in prep for H+E
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15
Q
- Give the 3 classifications of H+E and give3 examples
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16
Q
- Define – regressive and progressive staining, differentiation + give examples
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17
Q
- What is ‘blueing’?
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18
Q
- Give example of blueing agent
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19
Q
- Describe how to perform an H+E stain
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20
Q
- State the end result of H+E
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21
Q
- State precautions taken during reagent prep
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22
Q
- Name the 3 types of Mountant used in histo lab
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23
Q
- List the information displayed on a reagent label – state importance of these information
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