Lecture 4: embedding, microtomy Flashcards

1
Q

Define impregnation

A

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2
Q
  1. Define embedding
A

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3
Q
  1. What is the purpose of embedding
A

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4
Q
  1. List the methods of embedding
A

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5
Q
  1. What safety precautions must be taken while using an embedding centre
A

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6
Q
  1. What is the main function of a cryostat
A

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7
Q
  1. Give examples of types of embedding media
A

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8
Q
  1. Describe tissue orientation and explain the importance of correct tissue orientation
A

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9
Q
  1. List properties of embedding media
A

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10
Q
  1. Give advantages and disadvantages of a rotary microtome
A

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11
Q
  1. Name 4 types of knives
A

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12
Q
  1. Give 3 different knife profile and their uses
A

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13
Q
  1. Define microtomy
A

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14
Q
  1. What is the difference between serial and stepped section
A

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15
Q
  1. What is the ideal temperature for heating sections in an oven
A

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16
Q
  1. What is the ideal temperature of water bath for floating paraffin section. Why?
A

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17
Q
  1. What causes the following issues during microtomy:

o Scores in section

A

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18
Q
  1. What causes the following issues during microtomy:

o Holes in section

A

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19
Q
  1. What causes the following issues during microtomy:

o Compressed ribbons

A

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20
Q
  1. What causes the following issues during microtomy:

o Expansion of tissue on water bath

A

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21
Q
  1. What causes the following issues during microtomy:

o Unable to form ribbon

A

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