Lecture 4: embedding, microtomy Flashcards
1
Q
Define impregnation
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2
Q
- Define embedding
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3
Q
- What is the purpose of embedding
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4
Q
- List the methods of embedding
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5
Q
- What safety precautions must be taken while using an embedding centre
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6
Q
- What is the main function of a cryostat
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7
Q
- Give examples of types of embedding media
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8
Q
- Describe tissue orientation and explain the importance of correct tissue orientation
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9
Q
- List properties of embedding media
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10
Q
- Give advantages and disadvantages of a rotary microtome
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11
Q
- Name 4 types of knives
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12
Q
- Give 3 different knife profile and their uses
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13
Q
- Define microtomy
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14
Q
- What is the difference between serial and stepped section
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15
Q
- What is the ideal temperature for heating sections in an oven
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16
Q
- What is the ideal temperature of water bath for floating paraffin section. Why?
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17
Q
- What causes the following issues during microtomy:
o Scores in section
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18
Q
- What causes the following issues during microtomy:
o Holes in section
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19
Q
- What causes the following issues during microtomy:
o Compressed ribbons
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20
Q
- What causes the following issues during microtomy:
o Expansion of tissue on water bath
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21
Q
- What causes the following issues during microtomy:
o Unable to form ribbon
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